Need Help On Biology Course Work

brief: We are part of a company that is given tasks to do by clients within the areas of microbiology. We have been contacted by a company to look at 6 samples they have taken from areas where there seems to be microbial contamination We have been asked to determine the level of contamination, identify what organisms are contaminating each location and then provide advice as to any risks they pose and the best way to remove them. For each method used for identification we need to briefly explain it to the client and supply them with a resource for them to discover more. (a reference) Finally we each include in our report suggestions for improvements such as alterations to the methods used. You will need to provide a resource supporting this alteration  Client: Contamination at Bradshaw food processing plant As part of environmental monitoring we have been called upon to examine samples taken from various locations at a food processing plant in order to determine the source of a food spoilage organism.  Each sample was taken and resuspended in 10mls of phosphate buffered saline, and then sent to our company where it was then streaked directly onto TSA to provide each person with a water sample to enumerate, and a plate culture to use for identification and biofilm potential Locations  A Corner of warehouse, lightly soiled, low temperature (12oC) B Surface of hot pipe carrying steam, clean, high temperature (55oC) C Surface of wall near roof, cold temperatures, lightly soiled (10oC) D Café, Pipes over food preparation area. Lightly soiled, room temperature (21oC) E Café, Back of oven, high temperatures but cleaned (50oC) F Wash hand basin, clean, room temperature (21oC) Remember this just gives you an idea of what experiments you would have carried out during this week had we been in the labs, and therefore what experiments have been carried out for you by our technical staff. Enumeration. As well as identifying the microbes, the client also needs to know how contaminated each location is by finding the concentration of microbes in cells/ml. Note the microbial saline sample you are testing (A-F) on the provided Client report proforma.  Prepare a serial dilution of your microbial saline sample. Go down to a 10-4 dilution by preparing 10-fold serial dilutions as follows: · · · Add 900 mL of sterile distilled water to four 1.5 mL Eppendorf tubes and label 1 to 4. Add 100 mL of the sample you are testing to the tube labelled ‘1’ (10-1 dilution) and vortex briefly. · Transfer 100 mL from tube 1 to tube 2 (10-2 dilution). Vortex briefly. · Transfer 100 mL from tube 2 to tube 3 (10-3 dilution) and so on, to tube 4 (10-4 dilution). · To estimate Total Aerobic Counts,  inoculate 100 ml of each dilution including neat ( the 10ml microbial sample you were given), onto each TSA plate and spread using a sterile spreader.  Incubate plates at 37°C.   Client Project: Contamination of food processing locale. Location (E) Café, Back of oven, high temperatures but cleaned (50oC) a) Calculate the CFU/ml based on the data you were provided to complete the following table. Sample location: _________E_______________ Dilution Medium TSA Total number of colonies on plate Total CFU/ml Description of colony types observed Neat Too many to count Too many to count Number of colony is above 300, which means the colonies are all blurred together and it's very hard to count them correctly 10-1 Too many to count Too many to count Number of colony is above 300, which means the colonies are all blurred together and it's very hard to count them correctly 10-2 250 250000 cfu/ml 10-3 22 22000 cfu/ml Colony number below 30 which means there are too low in numbers and they are prone to contamination or error which can make big difference in the final numbers 10-4 2 2000 cfu/ml Number of colony is below 30 which means their are too low and they are prone to contamination or error which can make big difference in the final numbers State concentration of bacteria in the location showing your calculation The concentration in cfu/ml is 250000 Since it been diluted 100 fold we need to first do 250 x 100 = 25000 But we need the concentration in 1000ul, we must multiply the result by 10. Making it go from 100ul to 1000ul. 25000 x 10 = 250000 b) Identification of bacteria in location. State clearly, with photographic images, as evidence what you observed as a result of microscopic examination of gram staining, spore staining and oxidase tests. And any evidence from where the sample was taken. Remember to include brief explanation of each method to the client with at least one reference for them to find out more. State which API test you used and the results you observed including images of API strips and results you gained from the web page after sending code to Dr Loughlin I used API 50 CHB api® 50 CHB results Place a + or – in the box below the test. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 CONTROL GLY ERY DARA LARA RIB DXYL LXYL ADO MDX GAL GLU FRU MNE SBE RHA DUL INO MAN SOR MDM MDG NAG AMY ARB ESC + + - - + - - - - - - + + + - - - + + + - - + + + + 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 SAL CEL MAL LAC MEL SAC TRE INU MLZ RAF AMD GLYG XLT GEN TUR LYX TAG DFUC LFUC DARL LARL GNT 2KG 5KG + + + - - + + - - - - - - - - - - - - - - - - - And the web page gave me Show photographic images of biofilm staining experiment and comment on whether you feel this shows biofilm production or not. State what organism you have determined the sample to be and what health risks that might be associated with it. Include references to support your opinion. State your recommendation as to how to prevent the organism in the sample from continuing to contaminate the area sampled. Include references to support your opinion. Staff development: State any improvements or additions to the methodologies used and what benefits they would bring for the client or the company. You must include at least one reference to a resource, and you must provide evidence that you received feedback from a colleague on the suitability of the resource.