Kirby Bauer Statistics

Do My Homework Service Links: Online Assignment Help, Do My Assignments Online -

Please create data tables and graphs using the ANOVA statistical test. Use Excel sheets to perform the ANOVA Test. The Kirby Bauer Disk Diffusion method results are provided in the Excel sheet below.


Please only look at the tab titled "Kirby Bauer" that is all you need. Please do not include any N/A data! Use Excel to create the data set results. Analyze what these coyote scat bacterium results mean.


Also, please document the step by step process of how you are performing the ANOVA statistical test in Excel in a Word document so I too can learn and understand how do this test in Excel in the future.

Get Help With a similar task to - Kirby Bauer Statistics

Additional Instructions:

Nanodrop data _ Last Name email (csumb) Lab Time Unknown number Nanodrop ng/L Vicki Meraz vmeraz@csumb.edu 10:00 AM B-1 Nicole Diaz nidiaz@csumb.edu 10:00 AM B-2 Kylie Foley kfoley@csumb.edu 10:00 AM B-3 Victoria Valles vvalles@csumb.edu 10:00 AM B-4 Lily Westphal lwestphal@csumb.edu 2:00 PM B-6 Lauren Cooper lacooper@csumb.edu 12:00 PM B-7 Elizabeth Avalos eavalosarias@csumb.edu 12:00 PM B-8 Natalie Gil nagil@csumb.edu 12:00 PM B-9 Thomas Orozco thorozco@csumb.edu 2:00 PM B-11 Arabelle Franco arfranco@csumb.edu 2:00 PM B-12 Madeleine Hayman mhayman@gmail.com 2:00 PM B-13 Sam Boyd saboyd@scumb.edu 2:00 PM B-14 Nikki Arevalo marevalo@csumb.edu 2:00 B-15 Tristian Lozano tlozano@csumb.edu 2:00 PM B-16 Zell Newton znewton-gardington@csumb.edu 10:00 AM F-1 Leslie Pena lpena@csumb.edu 10:00 AM F-2 yasmine cachos ycachos@csumb.edu 10:00 AM F-3 Bruder Mei LIn mbruder@csumb.edu 12:00 PM F-6 Nicole Munoz nmunoz@csumb.edu 12:00 PM F-7 Caitlyn Swe cswe@csumb.edu 12:00:00 PM F-8 Fatima Leon-Garcia fleon-garcia@csumb.edu 12:00 PM F-9 Lilli Krier lkrier@csumb.edu 2:00 PM F-11 Erik Vaca evaca@csumb.edu 2:00 PM F-12 Cassandra Majano cmajano@csumb.edu 2:00 PM F-13 Jill Castro jicastro@csumb.edu 2:00 PM F-14 Ursula Lopez ulopez-blanca@csumb.edu 10:00 AM F-15 Allexxis Hand ahand@csumb.edu 2:00 PM F-16 Joseph Hanson jhanson@csumb.edu 2:00 PM M-1 Miranda Iturriaga miturriaga@csumb.edu 10:00 AM M-2 Michael Everton meverton@csumb.edu 10:00 AM M-3 Andrea Rios andrios@csumb.edu 10:00 AM M-4 Melissa Meraz memeraz@csumb.edu 12:00 PM M-7 Guadalupe Salamanca gsalamanca@csumb.edu 12:00 PM M-8 Marin Medina marimedina@csumb.edu 12:00 PM M-9 Natalie Ward nward@csumb.edu 2:00 PM M-11 Antonio ahernandez-sanchez@csumb.edu 2:00 PM M-12 Jakobia Brown Jakbrown@csumb.edu 10:00 AM M-13 Kendall DeRosa kderosa@csumb.edu 2:00 PM M-14 Carlos Rosas crosas@csumb.edu 14:00 M-15 Brianna Cabrera bcabrera@csumb.edu 2:00 PM T-1 Evan DesJardins 10:00 AM T-2 Peter Nelson pnelson@csumb.edu 10:00 AM T-3 Rachel Durand rdurand@csumb.edu 2:00 PM T-4 Alyssa Ruiz alyruiz@csumb.edu 10:00 AM T-5 Grace Fairbourn gfairbourn@csumb.edu 12:00 PM T-6 Raiya Kimble Rkimble@csumb.edu 12:00 PM T-7 Stephen Nelson Stnelson@csumb.edu 12:00 PM T-8 Nathan Gyan ngyan@csumb.edu 12:00 PM T-9 Roman Maaske rmaaske@csumb.edu 2:00 PM T-11 Mylisa King myking@csumb.edu 2:00 PM T-12 Alexa Blunt alblunt@csumb.edu 14:00 T-14 Kirby Bauer First Name Last Name Unknown Streptomycin Ampicillin Gentamycin Penicillin Cholamphenicol Tetracycline Neomycin Erythromycin CBD Oil Nicole Diaz B-2 22mm 12mm 22mm 6mm 20mm 12mm 24mm 26mm Guadalupe Salamanca M-8 15mm 21mm 21mm 0mm 26mm 18mm 20mm 9mm Joseph Hanson M-1 15mm 18mm 20mm 0mm 29mm 16mm 0mm 0mm Caitlyn Swe F-8 8mm 8mm 10mm 0mm 9mm 5mm 9mm 0mm Rachel Durand T-4 17mm 20mm 20mm 16mm 20mm 20mm 11mm 0mm Grace Fairbourn T-6 28mm N/A 27mm N/A 28mm 20mm 29mm 13mm N/A Brianna Cabrera T-1 15mm 18mm 29mm 0mm 27mm 20mm 0mm 0mm Kylie Foley B-3 24 mm 0 mm 23 mm 0 mm 18 mm 15 mm 23 mm 20 mm -- Lilli Krier F-11 5mm 6mm 10mm 0mm N/A 8mm N/A 0mm 0mm Madeleine Hayman B-13 9mm 10mm 12mm 0mm 11mm 9mm 11mm 4mm - Natalie Ward M-11 25mm N/A 27mm N/A 15mm 20mm 15mm Cassandra Majano F-13 17mm 21mm 22mm 0mm 16mm 20mm N/A 0mm 0mm Alexa Blunt T-14 19 mm 10 mm 28 mm 0 mm 20 mm 24 mm 25 mm 14 mm Erik Vaca F-12 25mm 11mm 28mm 8mm 25mm 11mm N/A 30mm 14mm Stephen Nelson T-8 20mm 8mm 24mm 0mm 15mm 19mm 27mm 14mm N/A Ursula Lopez F-15 20 mm 21 mm 22 mm 0 mm 25 mm 19 mm 22 mm 9 mm Mylisa King T-12 7 mm 9 mm 11 mm 0 mm 12 mm 9 mm N/A 4 mm 4 mm Raiya Kimble T-7 16 mm 25 mm 22 mm N/A 27 mm 23 mm 21 mm N/A N/A Mei Lin Bruder F-6 20 mm N/A 20 mm N/A 21 mm 19 mm 26 mm 17 mm N/A Sam Boyd B-14 14 mm 20 mm 20 mm 0 mm 25 mm 16 mm N/A 6 mm 0 mm Jakobia Brown M-13 16mm 16mm 23mm 0mm 27mm 17mm 24mm 0mm N/A Yasmine Cachos F-3 8 mm 10 mm 11 mm 0 mm 13 mm 12 mm 11 mm 0 mm Thomas Orozco B-11 11mm 13mm 12.5mm N/A 25mm 8mm 18mm N/A Fatima Leon-Garcia F-9 19 mm 22 mm 38mm 0mm 36 mm 21 mm 33mm 0mm N/A Lauren Cooper B-7 25 mm 10 mm 27 mm 0 mm 23 mm 13 mm 28 mm 28 mm N/A Zell Newton F-1 20 mm 17 mm 18mm 0mm 25 mm 19mm 24 mm 8 mm BiochemicalPhysical First Name Last Name email (csumb) Lab Time Unknown number Genus Species Percent ID Nanodrop ng/L Nucleotide Sequence Gram Stain Microscope Shape of bacteria Size of bacteria Anaerobic chamber growth? Y or N Na Thioglycolate Citrate Sulfur Reduction Indole (tryptophanase) Motility Starch (amylase) Gelatinase MR VP Endospore Present? (Y or N) Capsule Staining Nitrate result Oxidase Test MacConkey KIA Test Urease Catalase Vicki Meraz vmeraz@csumb.edu 10:00 AM B-1 Bacillus thuringiensis 98.82 GCAGTCGAGCGATGGATTGAGAGCTTGCTCTCAAGAAGTTAGCGGCGGAC GGGTGAGTAACACGTGGGTAACCTGTTCNTAAGACTGGGATAACTCCGGG AAACCGGGGCTAATACCGGATAACATTTTGAACTGCATGGTTCGAAATTG AAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTA GTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGA GGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG GCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGC CGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAG AACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAA GCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGC GTTATCCGGAATTATTGGGCGTAAAGCGCGCGCANGTGGTTTCTTAAGTC TGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAG ACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGC GTAGAGATATGGAGGAACACCAGTGGCGAANNCGACTTTTCTGGTCTGTA ACTGACACTGAGCGCGAAAGCGTGGGGAGCAAACAAGGATTAGATACCCT GGTAGTCCACGCC Gram + Bacillus Y Faculative areobe negative negative negative negative positive positive No Positive Nicole Diaz nidiaz@csumb.edu 10:00 AM B-2 Bacillus cereus 99.33% TGCAGTCGAGCGATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGNTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAGACTTGAGTGCAGAAGAAGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGANGCGCGAAAGCGTGGGGAGCAAACAGGATTA Gram + bacillus Y facultative anaerobe negative: The bacteria cannot use citrate as a carbon and energy source. negative:The microbe cannot reduce sulfer into ferrous ammonium sulfate and sodium thiosulfate to produse hydrogen sulfide. negative: The microbe does not produce indole from the amino acid tryptophan. positive: The medium appeared hazy which means it can swim using flagella. positive: The microbe can use starch as a complex carbohydrate from glucose as a source of canrbon and energy for growth. There was a halo currounding th colonies when iodine is added to plate. digest starch, positive for amylase. positive: bacteria has the gelatinase enzyme which allows the bacteria to breakdown gelatin in order to utilize the amino acids. The media liquified which indicates the presence of the gelatinase enzyme. N No capsule present Positive:The area where the colony touched turned black. The bacteria produces cytochrome c oxidase. The bacteria are capable of aerobic respiration and can use oxygen as a terminal electron acceptor in respiration. Positive: Bubbles appeared when hydrogen peroxide was added to the bacteria. the bacteria has the enzyme catalase to protect it from the toxic byproducts of oxygen metabolism. Kylie Foley kfoley@csumb.edu 10:00 AM B-3 Acinetobacter calcoaceticus GCAGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGANGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGNCTAACTCNGNGCCAGCAGCCGCGGTAATACAGANGGGTGCAAGCGTTAATCGGGATTTACTGGGCGTAAAGCGCGCGTANGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAA Gram - Cocci Y Facultative Anaerobe Positive: can use citrate as a carbon and energy source Negative: cannot reduce sulfur to sulfate/sulfide Negative: cannot make indole from tryptophan. Negative: no flagella. Negative: bacteria does not have amylase, cannot break down starch. Negative: does not have gelatinase enzyme, cannot break down to uses amino acids. N No capsule present Red after the addition of zinc=no nitrate reduction Negative: does not have cytrochrom c oxidase enzyme positive for growth (gram neg) and neg. for lactose fermenting (cannot produce lactose) Postitive: can breakdown H2O2. Victoria Valles vvalles@csumb.edu 10:00 AM B-4 Escherichia coli 99.13% AAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCCNGCGCGTTTGNTTAAGTCAGATGTGAAATCCCCGGGCTCNACCTGGGAACTGCATCTGATACTGGCAAGC Gram - Bacillus Y facultative anaerobe negative negative positive positive positive Negative N/A Red after the addition of A & B negative Pink growth Positive Lily Westphal lwestphal@csumb.edu 2:00 PM B-6 Acinetobacter calcoaceticus 99.35% GNNTNNGNNGCAGTCGAGCGGAGTGATGGTGCTTGCTCTATCACTTAGCGGCGGAGGGTGAGTNNGCTT AAGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGA TCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCT GTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGA GGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAG CCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATG TGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGT GTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAAGGCAGCCATCTGGCCTAACACTGACGCTGAGGTGC GAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCGTTGGGGCCTTTG AGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGA CGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAANCCTTACCTGGNCCTTGGACATAGT AAGAANTTTTNCCAGAGATGGNNTNGGNGNCCTTTCGGGNNNTTACATACNNNNCTGCATNGGNTGTCGNNCAGCNTCGN NNNCNNGANANNNNNNNNNNNNNNNNNNGNCAACNNNNCGCNNNCNTTNNNNNNNNNCNNNNNNNNANNNNNNNNNCTTT NNNN Gram - cocci 1 μm Y faculatative anarobe positive: bacteria can use citrate as a carbon and energy source negative: bacteria can not reduce sulfates to hydrogen sulfide negative- bacteria can not break tryptophan into indole and pyruvate positive: bacteria is motile Negative: bacteria does not have amylase. Can not break down starch Negative: bacteria does not have gelatinase. N No Capsule Present Negative- does notreduce nitrate Negative- bacteria does not contain cytochrome c oxidase enzyme Positive: bacteria is Gram negative and has the ability to ferment lactose Positive- bacteria has catalase. Is capable of catabolizing H2O2 into H20 + O2 Lauren Cooper lacooper@csumb.edu 12:00 PM B-7 Bacillus wiedmannii 99.83% GCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG Gram + Bacillus Y Facultative anaerobe Negative- Plate was green. Does not utilize citrate as a carbon source Negative- Medium was yellow/clear. No H2S production observed Negative- After adding Kovacs Reagent, no color change to red was observed in the medium. Bacteria can not breakdown tryptophan into indole and pyruvate Positive- Medium was cloudy and growth was not locatized to stab site. Bacteria is motile. Positive- After adding iodine to the plate, a halo was observed. Bacteria hydrolyzes starch by amylase enzyme. Positive- Medium turned from solid to liquid after 14 days. Gelatinase enzyme is present in the bacteria. Y No Capsule Present Positive- air bubble present in Durham tube. Nitrate gets reduced to N2 Positive- The test strip turned a dark color upon adding the bacteria. The bacteria has cytochrome c oxidase. Negative- The medium did not change color. The bacteria does not ferment lactose. There was no growth on the plate. The bacteria is gram positive. Positive- Bubbles formed after adding H2O2, bacteria has catalase enzyme. Is capable of breaking down H2O2 into H2O + O2 Elizabeth Avalos eavalosarias@csumb.edu 12:00 PM B-8 bacillus Y facultative anarobe Natalie Gil nagil@csumb.edu 12:00 PM B-9 Gam - Bacillus Y facultative anaerobe Negative Negative Positive Positive Negative Negative Negative Thomas Orozco thorozco@csumb.edu 2:00 PM B-11 Escherichia Escherichia fergusonii 97.91% CATGCAGTCGTACNNNGNANCNNNNNNNNNCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTGAG TAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAA GAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCACCTAGGC GACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACT TTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTG CCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG TCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAAT TCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCCTGGGACGAANACTGACG CTCANGTGCGAAANCGTGGGGGAGCAAACANNATTAGATACCCCTGGTAGTCCACGCCCGTAAACGATGTCGACTTGNNA GGTTGTGCCCCTNNGNNNNCGTGGCTTNCTNGNAGCTAATCNNCGTTNANNNNCGACCGCCCTGNGNGGAGGTAACTGGC CCGCCNANGNTNNNNAANTACNNNATGANTTTGNNNNGAGGGGGNNCNNNCCNCNANNNNGNNNGAGCATGNTGGNNTTT AATTTNNTATGCTANCGACGNNNANNNNTTNANCCCTNNNTNNNTNGNNNNNNNCNNNNNAANNNNNTNNNNNNAANGNA NNTANNNTNNNTTCNNNNNCNNNNNGAGACANNNNGNNNGGCNTGGNNTGGNCNGTCCNNNNNNNNNNTNNNNNAAATGN Gram - Bacillus Y facultative anaerobe negative negative positive positive negative negative Y No capsule Bubble at end of Durham tube, red after 8 drops of reagant a and b. Can reduce nitrate. Negative growth +, pink color change = ability to ferment lactose Arabelle Franco arfranco@csumb.edu 2:00 PM B-12 Acinetobacter Acinetobactor calcoaceticus 96.15% GNNNNCNTGNNAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTT AGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGA TCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCT GTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGA GGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAG CCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAATGT GAAATCCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGANAGGATGGTAGAATTCCNNGT GTAGCGGTGAAATGCGTANAGATCTGGANGAATACCGATGGCGAANGCNGCCATCTGGGCCTAACACTGACGCTGANTGC GAAANCATGGGGGAGCAAACNNGATTANATACCCTGGTAGTCCATGCCGNAAACGATGTCTACTANNCGNTTGGGGCCTC TTGTANGCTTTTNNGGCGCNGCTTANCNNNNNNAATANNAACNNNCCCTNNNGGAGTANNGGTCGCNNNAANNNNNAACT NNCNNANTGAANTTGNNCGNGGGGNCCNCCNCAAANCNGGGTGGGGAGCANNNNGNNNNAATTTCNNATNGNNNNNCNNC CNNNNAAAANNNTTNNNNTGGGCNNNGTNNNNNNNNNNTANNAANNNTTTCNNNAAANNNNNATNNNGTTGGGNNNTCT Gram - cocci Y Microaerophile positive negative negative negative positive negative Y Unknown Had a gas bubble but zinc powder turned red which means no nitrate reduction Negative Positive, enzyme can break down peroxide Madeleine Hayman mhayman@gmail.com 2:00 PM B-13 Escherichia fergusonii 96.99 CATGCAGTCGTACNNNGTAGCNCNGTGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAAT TCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGACGCT CNNTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTG CCCTTGAGGCGTGGCTTCCGGGANCTAACGCGTTAAGTCGACCGCCTGGGGANTACGNCCGCAANGTTTAAAACTCAAAT GAATTGACGGGGGCCCCGCCNCAAGCGGTGGGAGCATGNGGNTTNATTTCGATGCAACGCGAAGAANNNTTACNTNGGTC TTGACNTCCACNGNAANNTTTCNNNNATGAAAAATGNTGCCNTT Gram - bacilli Y Falcultative anaerobe neagtive negative positive positive positive negative N N positive Negative positive for growth (gram neg) and positive for lactose fermenting positive Sam Boyd saboyd@scumb.edu 2:00 PM B-14 E. coli 95.86 GNNNACNNTGCAGTCGTANNNNNNNNNGCNNNNNNNNCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTG AGTAATGTCTGGGAAACTGCCCGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCA AAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAG GCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC AGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTA CTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCG TGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTA AGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGA ATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACG CTCANGTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT TGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGGAGTANGGCCGCAANGGTTAAAACTC AAATGAATTTGACGGGGGGGCCCGCACAAGCGGGTGGGAGCATGGNGGTTTAATTTCGNATGCAACGCGAAAGAANCNNT ACCCTGGNNNTTGACATCCCACGGGAAGTTTTTCAGANNATGANNNNGNGCCNTTCGGNAANCNGTGANACAGGGTGCNN GCATNNNTGNCNNTCAGCCTCCNGTGNTGNGAAANGNTGGGNNNNNAANNNNCGN Gram - Bacillus Y Facultative Anaerobe Negative Negative Positive Positive Positive Negative Y N No nitrate reduction Negative Gram -, lactose fermentor Positive Nikki Arevalo marevalo@csumb.edu 14:00 B-15 Acinetobacter calcoaceticus 85.53 GNTTANAGTTNATGNNGNAGACNTTAGAGCTGAATCTNATGGCCTATCCTGGTAACGAACTATGA ACAAGGTATCCTGACAACATTGTCGAGTCTAAATGTGAATGCTAATCCAGGTAACCTTATACGGGAGAAAGCAGGCGATC TTCTGACCTTGCGCTAATAGATGAACCTAATTCAGACTTCTACTTGGAGGGGTACAGGCCTACCTAAGCGACNATCTGTA TCAGTTCGGAGAAGATGATACTCCNCTCTGGGACTGTTACGCAGCCCTTACTCCTACGAGACGCGGCNGTGGGGAAGATT GGACAGAGGGCGCAAGCCTGATTCAGCAATGCCGCGTGTGTGAAGAAGGCCTAATGGCTGTATAACACTTTNANCGAGGA GGAGGCTNCTGCCTTTAATACCTTANNNATAGTGGACGTTACTCNCAGAATAANCACCGGTATAACTNTNNGCCAGCAGC CGCGGTAATACAGAGGGTGCNAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAATGTG ANATCCCCCNAGCTTAACTTGGGAATTGCATTCNATACTGNTTAGCTAGAGTGTGGGAGANGATGGTANAATTTCTNNGT GTAGTCGGTGANATGCGTAGAGATCTGNNAGGAATANNNATGGTNGNNNGCNGCCCNTCTNGNNCTAATNACTGNCNNCT GANGTGCGANNNCATGNNGNAGNNAAAC Gram - Bracillus Y Obligate Aerobe Positive: Tristian Lozano tlozano@csumb.edu 2:00 PM B-16 gram - Bacillus Y facultative anaerobe negative negative positive positive negative negative N negative gram -, lactose fermentor positive Zell Newton znewton-gardington@csumb.edu 10:00 AM F-1 TGCAGTCGTACAAGGTAACCGTANNNGCTGCTGATCATGTGCGNCGCTGCNATGANNGNGNG GNTGATTNTNTTNNTAGAGAGNATTGATGTCGAGTNNCCNAACANAATCNNCGCCCCTTGGTTTTGCTCGGANNNAANAA GANACACTACNNGTNGGGCGTGGCCAATCACGCCCNGCCTTTTCACCAATCCCAGCACGCCCCNTTCCGGATCGCCGCCA CCGCGATAGCCACTACTCGAGACCGGCAGCAAATAGAGGGGCCCNGGGCTCACCNNTCTTTCTTGCTACNCCCTTACGCA AAAACGCCTTGNNANACTTCAATGATACATCTGGAGCGGAGAANGAATG Gram - Bacillus Y facultative anaerobe Negative Negative positive Positive positive Negative N/A No capsule present Had no bubble but turned red after the drops were added.Able to convert nitrate to nitrile negative growth, gram -, lactose fermenter positive Leslie Pena lpena@csumb.edu 10:00 AM F-2 Gram + cocci Y Facultative Anaerobe negative negative negative negative positive yasmine cachos ycachos@csumb.edu 10:00 AM F-3 Escherichia E.coli 94.81 GCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACC TAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGANGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGNTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAAGCGGCCCCCTGGGACGAAGACTGACGCTCNNTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTTCCACGCCCGTAAACGATGTCGACTTGGGANNTGTGCCCNTGAAGCGTGGCTTNCNNGCTAACGCGTTTAAGTCGACCGCCTGGGGAGTANGNCNNNNNGNTAAAACTCAAATGAANTGANNGNGGCCCNNCNCAAGCCGGTGGNNNCATGNNGTTTAATTCNATNGCAACNCGAANAAANCNTTNNNCCTNGTCTTT Gram - Bacillus Y Facultative anerobe negative negative positive positive negative negative N reduction of nitrate to nitrite; reduction of nitrate negative gram -, lactose fermenter positive Mei Lin Bruder mbruder@csumb.edu 12:00 PM F-6 Acinetobacter calcoaceticus 97.64 TATGCACTTAACCAGGGGGACCGGGAGAGTAATGCTTATGAATCTGCCTATTANTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTANCGGGTCTGANAGGATGATCCGCCACACTGGGACTGATACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTATAGCACTTTAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCCTATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAANGCAGCCATCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTANCCGTTGGGGGCCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGG Gram - Cocci Y Obligate Aerobe Positive Negative positive negative negative negative N N Clear after zinc added negative positive positive Nicole Munoz nmunoz@csumb.edu 12:00 PM F-7 Caitlyn Swe cswe@csumb.edu 12:00:00 PM F-8 Gram - Cocci (change to bacillus) Y Facultative Anaerobe negative negative positive positive negative negative negative positive Fatima Leon-Garcia fleon-garcia@csumb.edu 12:00 PM F-9 N/A sample was not good, did not get any hits with the nucleotide sequence obtained. N/A N/A CATGNAGTCGTACAAGGTANCCNGTANNNNGTATANNAAAGAGAAGGGTAGAAATAAAAAAANTTT GGGATTNNTNATTANAAAANNNGGGATTTAANGNNACGGCNCCCNNAATNCCCNCCNNNNNNTNAGCGN Gram - cocci Y Facultative Anaerobe negative, green indicating no change in pH, Cannot use citrate as a source of energy negative, can't reduce sulfur Positive, Bacteria can split amino acid tryptophan to form the compound indole Positive, there was growth in and around where it was stabbed negative, no halo was formed meaning the unknown bacteria can use starch as a source of carbon and energy for growth. negative, no liquid was formed on the surface, bacteria cannot produce gelatinase. N/A N/A N/A N/A Positive, indicating bacteria can reduce nitrate to nitrite Negative, bacteria does not produce cytochrome C oxidases Positive, pink/red indicating that the bacteria is lactose fermenting N/A N/A positve, a lot of bubbles present, bacteria produces catalase enzyme Lilli Krier lkrier@csumb.edu 2:00 PM F-11 Escheria E.coli GCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTG AGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCA AAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCACCTAG GCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC AGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAANGNCTTCGGGTTGTAAAGTA CTTTCNGCGGGGANGAANNGAGTAAAGNTAATACCTTTNCTCATTGACGTTTACCCCGCAGAAGAANCACCGGGCTAACT CCGTGCCAGCAGCCGCGGTAATACNGAGGGTGCGAGCGTTAATCGGAATTTACTGGGCGTAAAGCGCACGCACGCGGNTT GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTANNGGGGGG GAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCCTGGNACGAAGA CTGANNCTCNNTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTCNCACGCCCGTAAACGATGTCNACT TGGANTTGTGCCCTTGACGGCGTGNCTTCCCGGANCCTAACNCGTTAAGGTCGACCGCCTGGGGGANTNNGGGNCCGCNN GGTTAAAACTCAAAATGANATNNATNNGNNGGCCCCGNNNNNNNCCGNNGNAGCNTGNNGGNNNTTNNTTTNNAATGNNN ACGACGANNANNCATNAACCATGGNTNNTGNNNNTNCTNNCAGNANNNNNNANANNNTNNNNAAANGNNNNNCNTTCGGG NANNNNNGANANNNNGNCTNNCNNNNNNGTNCGNCCNNNCTCCNNGCT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive positive N/A(tube was lost) Durham tube= (+) for Nitrogen gas and (+) for Nitrate reducing to Nitrite Gram(-), lactase fermenting positive for catalase enzyme Erik Vaca evaca@csumb.edu 2:00 PM F-12 Bacillus wiedmannii 95.88% CTATACNTGCAGTCGAGCGATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTA ACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATG GTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTC ACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGA GGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGT AAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCT AACTACGTGCCAGCAGCCGCGGTAATACGTANGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGG TTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAGACTTGAGTGCAGAANAGG AAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAANGCGACTTTCTGGTCTGT AACTGACACTGAGGCGCGAAANCGTGGGGAGCAAACNNGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCT ANNTGTTANNNNNGTNTNCCGCCCCTTTTANTGCTGNAAGATTAATNGCATNNANGCACTCCTGCCNNGNNGGGAGTACN GGCCGCANNNCTGCANAACTNNNANNNATNGACGGGNGGGCNCCGCCACNNNNGNNGGNGCATGNNGGNNNTNAATTTNN NNAGCNNNNNCGANNNNNNTTNACTNANGNNNNTNNNCGATNCNTCTGCAAGAAACNCCNNNCNNANNATGANGNGNNNN NNNNNNNNNNNANNANNGAANNNNNNGNGCATN Gram + Cocci Y Facultative Anaerobe Negative negative negative negative negative postive Gas bubble present (+) ; Red color change (+) after addition of Reagents Positive Gram + , No lactose fermentation (-) positive for catalase enzyme Cassandra Majano cmajano@csumb.edu 2:00 PM F-13 Escheria E. fergusonii 95.76% ACATTGCAGTCGTANANNNNNNNNNNCNNNNNNNNCTTGCTTGCTTTGCTGACGAGTGGCGGACG GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAG ACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCAC CTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGG CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA AGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC TCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGG TAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACT GACGCTCNNTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGGA GGTTGTGCCCTTGNNNGTGGCTTCCCGGANCTAACGCGTTAAGTCGACCGCCTGGGGANTACGGCCGCNANGGTTAAAAC TCAAATGAATTGACGGGGGCCCCGCNNCAAGCNGNGGNANCATGNGNNTTAATTTCGATGCNACGCGNAAGAANNNTNAN CTGGGTCTTGACATCCACNGNAANNTTTTCANANATNGANNNNTGNTGCCCTTTCNGGNNNNNNNTNGANNAANNNNNGC NGGNCATNGGNNGTCGNNCANCNCNNNNT Gram - Bacillus Y facultative anaerobe Negative Negative Positive Positive Negative Negative Durham tube was postive for presence of Nitrogen gas and positive of reduction of NO2 Negative Gram (-) and is a Lactose Fermenter Positive for catalase enzyme Jill Castro jicastro@csumb.edu 2:00 PM F-14 Acinetobacter calcoaceticus 96.75% GCNNNCNTGNCNAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTANGAATCTGCCTATTANTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTANCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCANATAGTGGACGTTACTCNCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTANGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGANTTCCAGGTGTAGCGGTGAAATGCGTANAGATCTGGAGGAATACCGATGGCGAAAGCAGCCCTCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGGANNAAACAGGATTANATACCCTGGGTAGTCCNTGCCGTAAACGATGTCTACTANNNCGTTGGGGNCCTCTTNAGGCTTTTANTGGCGCNNCCTNNANNCGATATAATANAACNNNCCN Gram - Cocci Y Facultative Anaerobe Negative Ursula Lopez ulopez-blanca@csumb.edu 10:00 AM F-15 Escherichia E.coli 92.09% GCNANACATGCAGTCGTACAAGGTAGCCGTAAGNTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTNCNNGANGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCNTAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTANCTAGTAGGTGGGGTAACGGCTCACCAAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCATTGGNGAAGGGTGNAANNTGGGCGCAACCTGATGCCACCNTTCCGCGTGTATGAAAAANGCCTTCGGGTTGTATAGTACTTTTCAGCGGGGAGGAAGGGAGTACAATTAATA Gram - Bacillus Y Facultative Anaerobe: there was growth throughout the medium. This means that the bacteria can survive in high and low oxygen levels Negative: citrate agar plate did not have a color change (green). This means that the microbe was not able to use citrate for carbon and energy. The microbe did not accumulate alkaline/basic byproducts. Negative: no color change in the test tube. This means that the microbe was not able to reduce the sulfur in ferrous ammonium sulfate and sodium thiosulfate to produce hydrogen sulfide. Positive: color change at the top of the liquid in the test tube. This means that the microbe was able to produce indole from the amino acid tryptophan. Since indole was produced, the microbe reacted with a chemical reagent added after incubation to produce a color change. Positive: bacteria grew far away from the injection site and medium was hazy. This means that the bacteria was able to propel through the liquid by means of flagella. The microbe can swim by means of flagella. Negative: no halo in agar plate. This means that the microbe was not able to use starch as a source of carbon and energy for growth. Therefore, the microbe lacks the enzyme α-amylase Negative after 14 days: no liquification in the test tube. This means that the bacteria does not have the gelatinase enzyme. Since the bacteria does not have the gelatinase enzyme, it was not able to breakdown gelatin in order to utilize the amino acids. N No capsule present. There was a dark backrgound and purple stained bacterial cells were present. Positive: red after addition of A and B drops; no bubble present at top of Durham tube. Since the broth had a color change, it means that the unknown bacteria has denitifying capabilities and has a denitrifying enzyme. In the Durham tube there was no bubble which means that there was no reduction of N2. Negative: no color change on Sigma Aldrich strip. This means that the unknown bacteria does not contain the cytochrome c oxidase enzyme. Therefore, the unknown bacteria is not capable of aerobic respiration by having oxygen be the final electron acceptor in the electron transport chain. Gram - bacteria: pink growth on agar plate. This means that the bacteria was able to ferment lactose Negative: no bubbles on microscope slide. This means that the microbe does not have the catalase enzyme which is capable of catabolizing hydrogen peroxide into H2O and O2. Allexxis Hand ahand@csumb.edu 2:00 PM F-16 Escherichia fergusonii 95.98% CATGCAGTCGTANANNGNNNNGCGCNNNNNNNNGCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCNCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTC ACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGT AAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGT TTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTATAGATCTGGAGGAATACCGGTGGCGAAAGCGGCCCCCCTGGACGAAN ACTGACGCTCANGTGCGAAAGCGTGGGGNGCAAACACGNATTANATACCCCTGGNNAGTCCACGCCGTAAACGATGTCNACTTTGGNAGGTTGTGCCCCTTGNNNGTGGCTTCCGGANCTAACNCGTTNNNNCNNCCGCCTGGGGANTANNNCGCNNNNN NNNACTCTNATGNATTGACNGNGGGNCCCNCACAAGCNGNNGGGANNATGTGGNTTTNATTCGAATNGCNACNCGAANNNNNTTACCTNNNNTTTGNACATCNCACNNAANNTTT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive negative Negative after 14 days Possible endospores or it was just really burnt No Positive. After 8 drops of Reagant A and B it turned pink so bacteria can reduce nitrate into nitrite. Negative. Gram negative and lactose fermenting Positive Joseph Hanson jhanson@csumb.edu 2:00 PM M-1 NNNNNNNNNNNNNNNNNNNNNNNNNNANNGGGGTANNGNAAATAGTTTATTTTTTGAGNGGGNGGGGAGGTTCTTCTTTA TTGCCACTCCTNNNNAGACGCNNGACNGNNNNGCTACCCATNCCNTNTCCGCANAGACCNNNANGACTTCCTACGGNTCC TGACTCATGAGGCTNCCTGGGAGATTGTNGGNNTAGGCTANCNGNCCAAGANAATTGCNCCGNGGNATATGANAAGNACA CCNTCANGGTGAANTGACCCCCGGCCCACTCCCACGNCGNNAGCCGNGCGNGAAGTTTGGGACGGANGTTNNNNNNNTAC GANNNNCGATGNANNNNGAAAGGTTAGTGTNCATCTNCTGGGCCGAGGGACCGGGANGANTTTTCATTNNNTAANGGACG CCCGCANAAAAAGCCCTNCTTTTCTGCCCCCACNACANNTAGGANGCGTAATCTNTTTGTATTCTGAACTAAGCCNCCGG GAAN gram - cocci N facultative anaerobe negative, microbe cannot use citrate for carbon and energy negative, bacteria cannot reduce sulfur in ferrous ammonium sulfate / soidum thiosulfate to hydrogen sulfide positive, bacteria produces indole from tryptophan positive, bacteria can swim by flagella negative, bacteria cannot hydrolize starch negative, bacteria does not have gelatinase enzyme thus cannot break down gelatin or uitilize its amino acids Miranda Iturriaga miturriaga@csumb.edu 10:00 AM M-2 Acinetobacter Calcoaceticus 94.04% GGGGTCGATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTAYGAATCTGCCYMTTAGTGGGGGACAACATTTCGAARGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAKACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACGTTTACTCGCARAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAAWTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTATARATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAACAACTGACGCTGAAGGTGGCGGAA Gram - Cocci N Obligated Aerobic Positive Negative Negative Negative Negative Negative Negative Negative Gram -/lactose fermentor Positive Michael Everton meverton@csumb.edu 10:00 AM M-3 Acinetobacter Lactucae GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram + Bacillus N Faculatative Aerobic positive negative negative negative N Andrea Rios andrios@csumb.edu 10:00 AM M-4 Acinetobacter Acinetobacter calcoaceticus GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram - cocci Melissa Meraz memeraz@csumb.edu 12:00 PM M-7 Acinetobacter GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram - Cocci Y Facultative anaerobe Positive Negative Negative Positive Negative Negative N N Negative Negative Gram - and lactose fermentor Positve Guadalupe Salamanca gsalamanca@csumb.edu 12:00 PM M-8 NNNNNNNGNNACNNTGCAGTCGTACAGGTANCCGTANNTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCT Gram - Bacillus Y Facultative Anaerobic Negative Negative Positve Positive Negative Negative Negative Positve Positive Marin Medina marimedina@csumb.edu 12:00 PM M-9 Escherichia E. Coli 97.32% GGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA (ACCNTGCAGTCGTACAGGTAGCCGTAACTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCT GGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA CCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCC CTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGG GGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGC CGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGT GAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG TAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCG AAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGA GGCGTGGCNTTCCGGANCTAACGCGTTAAGTCGACCGCCCTGGGGGAGTACNNCCGCAANGTTTAAAACTCNNATGAATT TGACGGGGGGCCCCGCCNCNAAGCCGGNNGGANCATGTGGNTTAATTTCNATGC) Gram - Bacillus Y Facultative anaerobe negative negative negative positive positive Negative N No Capsule present Negative Natalie Ward nward@csumb.edu 2:00 PM M-11 Acinetobacter sp. calcoaceticus 90.79% CCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCC gram - cocci Y facultative anaerobe positive (blue) negative, no H2S produced negative negative negative Negative; cannot digest gelatin for carbon and energy source N No Capsule present Positive; Bubble in durham tube(possible nitrate), no color change after reagents, red after addition of zinc (no nitrate reduction) Negative Positive Gram - growth and Lactose fermentation Positive for catalase enzyme Antonio Hernandez ahernandez-sanchez@csumb.edu 2:00 PM M-12 Acinetobacter calcoaceticus 97.29% GCAAGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTA Y Facultative anaerobe Positive(blue color) negative negative negative negative Negative Positive Jakobia Brown Jakbrown@csumb.edu 10:00 AM M-13 Escherichia E.coli 86.82% GCNNNNCATGCAAGTCGTACNNNNNNNNNCNNNNNNNNNCTTGCTGCTTTGCTGACGAGTGGCGGACGGGT GAGTAATGTCTGGGAAACTGCCCGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACC AAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTA GGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAG CAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT ACTTTCAGCGGGGGAGGGAAGGGGANNTAAAGTTAATANCCTTTTGCCTCATTGGNACGTTNCCCCGNNAGAAANAAGGC ACACCGGCTNNACTCTCCNNTCCNNNCAAGCCCGNCGGTAAATANCNGGAGAGGTGTGCNAGCCGTTAATCNGGAAANTN NNNGGGGCNNNAAAANCNNCNCNCNNGGCGGGTTTGTTAAGTCAGAATGTGAAATCCCCCCGGGCTTCANCCTGGGGAAC TGCCTCNTGATACTGGGCNAGCTTGAGTCTCCGTAGANGGGGGGNAGAATTCCCNGGTGTTAGCGGTGNNAATGCGTANA NATCTGGGAGGAATACCGGNGGNGAANGCGGCCCCCTGGNCGAAANANTGACGCTCNNGTGCGNAAANNGTGNNGGAGGC AAACANGGATTANATNACCCCTNNGNNAGTCCACGCCCGCNNNANNANGNTCNNACNTAGTGANGGNTGTTGTCCCCNNN NNNNGCGGNNNNTGTCCNNGNNNNNNNAACNNNNNTNNNNNNTNCNNCCNNNCCCTGNNNNGNANNNNNNNNNCCCNNCC NNNNNNTNNNNNANCNTNCNCNANNNNANNNNNNNNNGGNGGNNNNNNNNNNNNGNNNGGNNNNANNNNGNNNNNNTNNN NTGNNANNNNCNNANNNNNNTNCNTNGNNNNNNNNNNNNNCCNNCNNNCANNNNTTC Gram - Bacillus Y Facultative Anaerobe Negative (Green) Negative Positve positive Positive Negative N/A N/a Positive: The tube had a bubble so it is a denitrifying bacteria Positive: uses oxygen as the final electron acceptor Gram negatvie and was pink meaning that it has lactose fermenting colonies Positive- Bacteria bubbled Kendall DeRosa kderosa@csumb.edu 2:00 PM M-14 AGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGA Carlos Rosas crosas@csumb.edu 14:00 M-15 CGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTG Gram - Bacillus Y Facultative anaerobe negative negative negative negative negative positive Brianna Cabrera bcabrera@csumb.edu 2:00 PM T-1 Shigella S. dysenteriae 92.01% GGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive negative negative N Able to reduce Nitrate to Nitrite Negative Gram (-), ability to ferment lactose Negative Evan DesJardins edesjardins@csumb.edu 10:00 AM T-2 AAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGC Gram + Bacillus Y facultatice anaerobe negative negative positive positive negative negative Positive Gram-,lactase Peter Nelson pnelson@csumb.edu 10:00 AM T-3 CAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGT Gram - cocci Y Facultative anaerobe negative Rachel Durand rdurand@csumb.edu 2:00 PM T-4 Acinetobacter calcoaceticus 96.58% CAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATT Gram - Bacillus Obligate Aerobe negative negative positive positive negative negative Negative Positive Alyssa Ruiz alyruiz@csumb.edu 10:00 AM T-5 CCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAANNCAGCCATCTGGCCTAACACTGACGCTG Gram - Cocci Y Facultative Anaerobe negative negative positive positive negative- unable to digest starch for carbon and energy source negative- no gelatinase enzyme, unable to use gelatin as carbon and energy source NA- was unable to complete test NA- unreadable under the microscope positive- bacteria has denitrifying capabilities, reduction to N2 Negative positive, gram - and can ferment lactose negative- not capable of catabolizing hydrogen peroxide Grace Fairbourn gfairbourn@csumb.edu 12:00 PM T-6 Acinetobacter calcoaceticus ANTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCCGTTGGGG gram - cocci Y - can survive without oxygen Facultative Anaerobe - can survive in high and low oxygen positive - can utilize citrate as carbon and energy negative - cannot reduce sulfur negative - does not produce indole from trypotophan negative - cannot freely move around negative - cannot digest starch for source of carbon and energy negative - cannot digest gelatin for carbon and energy source negative - cannot reduce nitrate at all Negative - does not have cytocrome C oxidase enzyme lactose fermenting gram - positive - can safely breakdown H2O2 Raiya Kimble Rkimble@csumb.edu 12:00 PM T-7 NCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGGAGTACNGTCGCANACTAAAACTCAAATGA gram - Bacillus Facultative anaerobic- no growth at the top of broth negative negative Positive Positive positive Negative Negative Positive, gram - Stephen Nelson Stnelson@csumb.edu 12:00 PM T-8 Acinetobacter lactucae 93.59% ANTTGACGGGGGNCCNCCNCNAGCGGNGGNNCATGNNGNTTAATTCGATGCNNCGCGAANAANNTTANCNNGG Gram - Cocci 0.5 micrometer diameter Y Facultative Anaerobe Positive Negative Positive Positive Negative Negative after 14 days No endospores evident in staining - Also, species is cocci which are not known to produce endospores No capsule present Negative Lactose-fermenting, Gram - Positive for catalase production Nathan Gyan ngyan@csumb.edu 12:00 PM T-9 Escherichia Escherichia coli 92.35% TGMAKTCGDAVRRGGTAGSCGTAACTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACCTAGGCGACGAYCCCTASCTGGTCTGAGAGGWKGACCAGCCACACTGGAACTGARACACGGTCCARACTCCTACGGGAGGCAGCAKTGGGGAATWTTGCMCAATGGGCGCAAGCCTGWDGCASCCWTGCCGCGTGTATGAARAAGGSCTTCGGGTTGDAAAGTACTTTCVGCGGGGARGAAGGGARDAAAGKTAATACCTTTGCTCHTTGACGTTHCCCGCARAARAAGCACCGGCTAACTCCGKGCCAGCAGCCGCGGTAATACGGAGGGKGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCMCGCAGGCGGTTTGTTAADTCAGAWGTGAAATCCCCGGGCTCAACCTGGGAACTGCHTCTGATACTGGCAAGCTTGAGTCTCGTARARGGGGGKAGAATTCCVGGKGTAGCGGKGAAATGCGTARAGATCTGGAGGAATACCGGBGGCGAAGGCGGCCCCCTGSACGAANACTGACGCTCANGTGCGAAAGCGTGGGGGAGCAAACAGGATTANATACCCTNGNTAGTCCMCGCCGTAAACVATGTCGACTTGGAGGTTGTGCCCTTGANGCGTGGCTTCCGGNAGCTAACGCNTTTAAGTCGACCGCCTGGGGGAGGTANGNCCGCAAGGTTAAAACTCAAATGAATTTGACTGGGGGGCCCNGCCNCAAGCCGGTGGANCNTGTNNTTTAATTTCGATGGCAA Gram - Cocci Y Facultative Anaerobe Negative Negative Positive Positive Negative Negative Negative Roman Maaske rmaaske@csumb.edu 2:00 PM T-11 Gram- Bacillus Y facultative anaerobe Mylisa King myking@csumb.edu 2:00 PM T-12 CNNTGCAGTCGTANANNNNNNNNGCNNGTNNAGCTTGCTGCTTTGCTGACGAGTGGCGGACGGG TGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGAC CAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACCT AGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA GCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG TACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTC CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGT TAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTA GAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGA CGCTCNGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGGAGG TTGTGCCCTTGANNCGTGGGCTTCCCGGANCTAACGCGTTAAGTCGACCCGCCCTGGGGAGTANGNCCGCAAGGTTAAAA CTCAAATGAATTTGACGGGGGNCCCGCCNCAAGNCGGTGGANCATGGNGGNNNATTTCGATGCNNNCNCGAANAANNNTT ACCTNGTCNTGNCNTCCANNNNNGTTTTCNNANATGNNAATGTGNCNTTNNNNNNNCNNNGANNANNNNNNNNNGCATNN NTNNTCGTCAGCCTCC Gram - bacillus Y Facultative anaerobe Negative Negative Positive Positive Negative Negative @ day 7 Y Not present 3 bubbles, Red after Reagent A & B Negative Lacatose fermentor (pink in color), Gram- negative (growth present) Positive Alexa Blunt alblunt@csumb.edu 14:00 T-14 Acinetobacter Acinetobacter lactucae 97.81% ACATGCAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGAGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGAGGCTACTTTAGATAATACCTAGAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGNNTGCGTAGAGATCTGGAGGAATACCGATGGCGAAGGCAGCCATCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCGTTGGGGCCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGANCATGNNGGTTTAATTTCGATGCANCGCGAAGAANCCTTTANCCTGGNCNTGACNTANTAAGAACTTTTCCCANNANNATGGGAATTGGNGNNTTNNNNNNTTACATACAGNGCTGCATGNTGTCGTCNNCTCNNGNCGNGANAT Gram - cocci Y Facultative Anaerobe Positive Negative Negative Negative Negative Negative after 14 days Possible endospore, green stained Negative Color change after gas bubble present and addition of Reagents A and B Negative Negative Lactose fermentation, pink with no pink cloud, positive Kendall DeRosa kderosa@csumb.edu 2:00 PM m-16 Acinetobacter Acinetobacter calcoaceticus 93.15 TNACCNTGCNGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGGATCCAGCCATGCCGCGTGTGTGAAGAAANNCTTATGGNTGTAAAGCACTTTAAGCGAGGANGANGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCANAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGANGGTGCAAGCGTTAATCNGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAANGTGAAATCCCCNAGCTTAACTTGGGGAATTGCATTCGATACTGGTTAGCTAGANTGTGGGGAGANGATGGTAGAATTCCANGTGTAGCGGTGAAATGCGTAGAGATCTGGANGAATACCGATGGCGAAAGCAGCCATCTGGGCCTAACACTGACNCTGAGTGCGAAAGCATGGGGAGCAAACANGGATTAGATACCCTGGGTAGTCCATGCCCGTAAACGATGTCTACTANCCNTTTGGGGCCTTTGNNNCTTTANTGGCGCAGCCNAACNCCNATNAAGTAANAACCGCCNNGGGGGCANGTACGGGTTCGCNNNACTAAAACTCNAATNNANTTGANNGGGGGNCCCGCCNNCNNNCCNNTGGNANCATGNTGNNTTTAATTTCNATNNNNNNCCNCNNNNAACCNNNANCCTNGNNNNTGNCNTNNGTAANNANNTNNNNNANNNNNNNNNNNNNNCGGAACTTNNANTNCNGNNCCTGCAT Gram -

Nanodrop data _ Last Name email (csumb) Lab Time Unknown number Nanodrop ng/L Vicki Meraz vmeraz@csumb.edu 10:00 AM B-1 Nicole Diaz nidiaz@csumb.edu 10:00 AM B-2 Kylie Foley kfoley@csumb.edu 10:00 AM B-3 Victoria Valles vvalles@csumb.edu 10:00 AM B-4 Lily Westphal lwestphal@csumb.edu 2:00 PM B-6 Lauren Cooper lacooper@csumb.edu 12:00 PM B-7 Elizabeth Avalos eavalosarias@csumb.edu 12:00 PM B-8 Natalie Gil nagil@csumb.edu 12:00 PM B-9 Thomas Orozco thorozco@csumb.edu 2:00 PM B-11 Arabelle Franco arfranco@csumb.edu 2:00 PM B-12 Madeleine Hayman mhayman@gmail.com 2:00 PM B-13 Sam Boyd saboyd@scumb.edu 2:00 PM B-14 Nikki Arevalo marevalo@csumb.edu 2:00 B-15 Tristian Lozano tlozano@csumb.edu 2:00 PM B-16 Zell Newton znewton-gardington@csumb.edu 10:00 AM F-1 Leslie Pena lpena@csumb.edu 10:00 AM F-2 yasmine cachos ycachos@csumb.edu 10:00 AM F-3 Bruder Mei LIn mbruder@csumb.edu 12:00 PM F-6 Nicole Munoz nmunoz@csumb.edu 12:00 PM F-7 Caitlyn Swe cswe@csumb.edu 12:00:00 PM F-8 Fatima Leon-Garcia fleon-garcia@csumb.edu 12:00 PM F-9 Lilli Krier lkrier@csumb.edu 2:00 PM F-11 Erik Vaca evaca@csumb.edu 2:00 PM F-12 Cassandra Majano cmajano@csumb.edu 2:00 PM F-13 Jill Castro jicastro@csumb.edu 2:00 PM F-14 Ursula Lopez ulopez-blanca@csumb.edu 10:00 AM F-15 Allexxis Hand ahand@csumb.edu 2:00 PM F-16 Joseph Hanson jhanson@csumb.edu 2:00 PM M-1 Miranda Iturriaga miturriaga@csumb.edu 10:00 AM M-2 Michael Everton meverton@csumb.edu 10:00 AM M-3 Andrea Rios andrios@csumb.edu 10:00 AM M-4 Melissa Meraz memeraz@csumb.edu 12:00 PM M-7 Guadalupe Salamanca gsalamanca@csumb.edu 12:00 PM M-8 Marin Medina marimedina@csumb.edu 12:00 PM M-9 Natalie Ward nward@csumb.edu 2:00 PM M-11 Antonio ahernandez-sanchez@csumb.edu 2:00 PM M-12 Jakobia Brown Jakbrown@csumb.edu 10:00 AM M-13 Kendall DeRosa kderosa@csumb.edu 2:00 PM M-14 Carlos Rosas crosas@csumb.edu 14:00 M-15 Brianna Cabrera bcabrera@csumb.edu 2:00 PM T-1 Evan DesJardins 10:00 AM T-2 Peter Nelson pnelson@csumb.edu 10:00 AM T-3 Rachel Durand rdurand@csumb.edu 2:00 PM T-4 Alyssa Ruiz alyruiz@csumb.edu 10:00 AM T-5 Grace Fairbourn gfairbourn@csumb.edu 12:00 PM T-6 Raiya Kimble Rkimble@csumb.edu 12:00 PM T-7 Stephen Nelson Stnelson@csumb.edu 12:00 PM T-8 Nathan Gyan ngyan@csumb.edu 12:00 PM T-9 Roman Maaske rmaaske@csumb.edu 2:00 PM T-11 Mylisa King myking@csumb.edu 2:00 PM T-12 Alexa Blunt alblunt@csumb.edu 14:00 T-14 Kirby Bauer First Name Last Name Unknown Streptomycin Ampicillin Gentamycin Penicillin Cholamphenicol Tetracycline Neomycin Erythromycin CBD Oil Nicole Diaz B-2 22mm 12mm 22mm 6mm 20mm 12mm 24mm 26mm Guadalupe Salamanca M-8 15mm 21mm 21mm 0mm 26mm 18mm 20mm 9mm Joseph Hanson M-1 15mm 18mm 20mm 0mm 29mm 16mm 0mm 0mm Caitlyn Swe F-8 8mm 8mm 10mm 0mm 9mm 5mm 9mm 0mm Rachel Durand T-4 17mm 20mm 20mm 16mm 20mm 20mm 11mm 0mm Grace Fairbourn T-6 28mm N/A 27mm N/A 28mm 20mm 29mm 13mm N/A Brianna Cabrera T-1 15mm 18mm 29mm 0mm 27mm 20mm 0mm 0mm Kylie Foley B-3 24 mm 0 mm 23 mm 0 mm 18 mm 15 mm 23 mm 20 mm -- Lilli Krier F-11 5mm 6mm 10mm 0mm N/A 8mm N/A 0mm 0mm Madeleine Hayman B-13 9mm 10mm 12mm 0mm 11mm 9mm 11mm 4mm - Natalie Ward M-11 25mm N/A 27mm N/A 15mm 20mm 15mm Cassandra Majano F-13 17mm 21mm 22mm 0mm 16mm 20mm N/A 0mm 0mm Alexa Blunt T-14 19 mm 10 mm 28 mm 0 mm 20 mm 24 mm 25 mm 14 mm Erik Vaca F-12 25mm 11mm 28mm 8mm 25mm 11mm N/A 30mm 14mm Stephen Nelson T-8 20mm 8mm 24mm 0mm 15mm 19mm 27mm 14mm N/A Ursula Lopez F-15 20 mm 21 mm 22 mm 0 mm 25 mm 19 mm 22 mm 9 mm Mylisa King T-12 7 mm 9 mm 11 mm 0 mm 12 mm 9 mm N/A 4 mm 4 mm Raiya Kimble T-7 16 mm 25 mm 22 mm N/A 27 mm 23 mm 21 mm N/A N/A Mei Lin Bruder F-6 20 mm N/A 20 mm N/A 21 mm 19 mm 26 mm 17 mm N/A Sam Boyd B-14 14 mm 20 mm 20 mm 0 mm 25 mm 16 mm N/A 6 mm 0 mm Jakobia Brown M-13 16mm 16mm 23mm 0mm 27mm 17mm 24mm 0mm N/A Yasmine Cachos F-3 8 mm 10 mm 11 mm 0 mm 13 mm 12 mm 11 mm 0 mm Thomas Orozco B-11 11mm 13mm 12.5mm N/A 25mm 8mm 18mm N/A Fatima Leon-Garcia F-9 19 mm 22 mm 38mm 0mm 36 mm 21 mm 33mm 0mm N/A Lauren Cooper B-7 25 mm 10 mm 27 mm 0 mm 23 mm 13 mm 28 mm 28 mm N/A Zell Newton F-1 20 mm 17 mm 18mm 0mm 25 mm 19mm 24 mm 8 mm BiochemicalPhysical First Name Last Name email (csumb) Lab Time Unknown number Genus Species Percent ID Nanodrop ng/L Nucleotide Sequence Gram Stain Microscope Shape of bacteria Size of bacteria Anaerobic chamber growth? Y or N Na Thioglycolate Citrate Sulfur Reduction Indole (tryptophanase) Motility Starch (amylase) Gelatinase MR VP Endospore Present? (Y or N) Capsule Staining Nitrate result Oxidase Test MacConkey KIA Test Urease Catalase Vicki Meraz vmeraz@csumb.edu 10:00 AM B-1 Bacillus thuringiensis 98.82 GCAGTCGAGCGATGGATTGAGAGCTTGCTCTCAAGAAGTTAGCGGCGGAC GGGTGAGTAACACGTGGGTAACCTGTTCNTAAGACTGGGATAACTCCGGG AAACCGGGGCTAATACCGGATAACATTTTGAACTGCATGGTTCGAAATTG AAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTA GTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGA GGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG GCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGC CGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAG AACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAA GCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGC GTTATCCGGAATTATTGGGCGTAAAGCGCGCGCANGTGGTTTCTTAAGTC TGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAG ACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGC GTAGAGATATGGAGGAACACCAGTGGCGAANNCGACTTTTCTGGTCTGTA ACTGACACTGAGCGCGAAAGCGTGGGGAGCAAACAAGGATTAGATACCCT GGTAGTCCACGCC Gram + Bacillus Y Faculative areobe negative negative negative negative positive positive No Positive Nicole Diaz nidiaz@csumb.edu 10:00 AM B-2 Bacillus cereus 99.33% TGCAGTCGAGCGATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGNTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAGACTTGAGTGCAGAAGAAGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGANGCGCGAAAGCGTGGGGAGCAAACAGGATTA Gram + bacillus Y facultative anaerobe negative: The bacteria cannot use citrate as a carbon and energy source. negative:The microbe cannot reduce sulfer into ferrous ammonium sulfate and sodium thiosulfate to produse hydrogen sulfide. negative: The microbe does not produce indole from the amino acid tryptophan. positive: The medium appeared hazy which means it can swim using flagella. positive: The microbe can use starch as a complex carbohydrate from glucose as a source of canrbon and energy for growth. There was a halo currounding th colonies when iodine is added to plate. digest starch, positive for amylase. positive: bacteria has the gelatinase enzyme which allows the bacteria to breakdown gelatin in order to utilize the amino acids. The media liquified which indicates the presence of the gelatinase enzyme. N No capsule present Positive:The area where the colony touched turned black. The bacteria produces cytochrome c oxidase. The bacteria are capable of aerobic respiration and can use oxygen as a terminal electron acceptor in respiration. Positive: Bubbles appeared when hydrogen peroxide was added to the bacteria. the bacteria has the enzyme catalase to protect it from the toxic byproducts of oxygen metabolism. Kylie Foley kfoley@csumb.edu 10:00 AM B-3 Acinetobacter calcoaceticus GCAGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGANGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGNCTAACTCNGNGCCAGCAGCCGCGGTAATACAGANGGGTGCAAGCGTTAATCGGGATTTACTGGGCGTAAAGCGCGCGTANGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAA Gram - Cocci Y Facultative Anaerobe Positive: can use citrate as a carbon and energy source Negative: cannot reduce sulfur to sulfate/sulfide Negative: cannot make indole from tryptophan. Negative: no flagella. Negative: bacteria does not have amylase, cannot break down starch. Negative: does not have gelatinase enzyme, cannot break down to uses amino acids. N No capsule present Red after the addition of zinc=no nitrate reduction Negative: does not have cytrochrom c oxidase enzyme positive for growth (gram neg) and neg. for lactose fermenting (cannot produce lactose) Postitive: can breakdown H2O2. Victoria Valles vvalles@csumb.edu 10:00 AM B-4 Escherichia coli 99.13% AAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCCNGCGCGTTTGNTTAAGTCAGATGTGAAATCCCCGGGCTCNACCTGGGAACTGCATCTGATACTGGCAAGC Gram - Bacillus Y facultative anaerobe negative negative positive positive positive Negative N/A Red after the addition of A & B negative Pink growth Positive Lily Westphal lwestphal@csumb.edu 2:00 PM B-6 Acinetobacter calcoaceticus 99.35% GNNTNNGNNGCAGTCGAGCGGAGTGATGGTGCTTGCTCTATCACTTAGCGGCGGAGGGTGAGTNNGCTT AAGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGA TCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCT GTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGA GGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAG CCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATG TGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGT GTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAAGGCAGCCATCTGGCCTAACACTGACGCTGAGGTGC GAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCGTTGGGGCCTTTG AGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGA CGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAANCCTTACCTGGNCCTTGGACATAGT AAGAANTTTTNCCAGAGATGGNNTNGGNGNCCTTTCGGGNNNTTACATACNNNNCTGCATNGGNTGTCGNNCAGCNTCGN NNNCNNGANANNNNNNNNNNNNNNNNNNGNCAACNNNNCGCNNNCNTTNNNNNNNNNCNNNNNNNNANNNNNNNNNCTTT NNNN Gram - cocci 1 μm Y faculatative anarobe positive: bacteria can use citrate as a carbon and energy source negative: bacteria can not reduce sulfates to hydrogen sulfide negative- bacteria can not break tryptophan into indole and pyruvate positive: bacteria is motile Negative: bacteria does not have amylase. Can not break down starch Negative: bacteria does not have gelatinase. N No Capsule Present Negative- does notreduce nitrate Negative- bacteria does not contain cytochrome c oxidase enzyme Positive: bacteria is Gram negative and has the ability to ferment lactose Positive- bacteria has catalase. Is capable of catabolizing H2O2 into H20 + O2 Lauren Cooper lacooper@csumb.edu 12:00 PM B-7 Bacillus wiedmannii 99.83% GCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG Gram + Bacillus Y Facultative anaerobe Negative- Plate was green. Does not utilize citrate as a carbon source Negative- Medium was yellow/clear. No H2S production observed Negative- After adding Kovacs Reagent, no color change to red was observed in the medium. Bacteria can not breakdown tryptophan into indole and pyruvate Positive- Medium was cloudy and growth was not locatized to stab site. Bacteria is motile. Positive- After adding iodine to the plate, a halo was observed. Bacteria hydrolyzes starch by amylase enzyme. Positive- Medium turned from solid to liquid after 14 days. Gelatinase enzyme is present in the bacteria. Y No Capsule Present Positive- air bubble present in Durham tube. Nitrate gets reduced to N2 Positive- The test strip turned a dark color upon adding the bacteria. The bacteria has cytochrome c oxidase. Negative- The medium did not change color. The bacteria does not ferment lactose. There was no growth on the plate. The bacteria is gram positive. Positive- Bubbles formed after adding H2O2, bacteria has catalase enzyme. Is capable of breaking down H2O2 into H2O + O2 Elizabeth Avalos eavalosarias@csumb.edu 12:00 PM B-8 bacillus Y facultative anarobe Natalie Gil nagil@csumb.edu 12:00 PM B-9 Gam - Bacillus Y facultative anaerobe Negative Negative Positive Positive Negative Negative Negative Thomas Orozco thorozco@csumb.edu 2:00 PM B-11 Escherichia Escherichia fergusonii 97.91% CATGCAGTCGTACNNNGNANCNNNNNNNNNCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTGAG TAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAA GAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCACCTAGGC GACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACT TTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTG CCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG TCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAAT TCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCCTGGGACGAANACTGACG CTCANGTGCGAAANCGTGGGGGAGCAAACANNATTAGATACCCCTGGTAGTCCACGCCCGTAAACGATGTCGACTTGNNA GGTTGTGCCCCTNNGNNNNCGTGGCTTNCTNGNAGCTAATCNNCGTTNANNNNCGACCGCCCTGNGNGGAGGTAACTGGC CCGCCNANGNTNNNNAANTACNNNATGANTTTGNNNNGAGGGGGNNCNNNCCNCNANNNNGNNNGAGCATGNTGGNNTTT AATTTNNTATGCTANCGACGNNNANNNNTTNANCCCTNNNTNNNTNGNNNNNNNCNNNNNAANNNNNTNNNNNNAANGNA NNTANNNTNNNTTCNNNNNCNNNNNGAGACANNNNGNNNGGCNTGGNNTGGNCNGTCCNNNNNNNNNNTNNNNNAAATGN Gram - Bacillus Y facultative anaerobe negative negative positive positive negative negative Y No capsule Bubble at end of Durham tube, red after 8 drops of reagant a and b. Can reduce nitrate. Negative growth +, pink color change = ability to ferment lactose Arabelle Franco arfranco@csumb.edu 2:00 PM B-12 Acinetobacter Acinetobactor calcoaceticus 96.15% GNNNNCNTGNNAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTT AGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGA TCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCT GTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGA GGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAG CCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAATGT GAAATCCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGANAGGATGGTAGAATTCCNNGT GTAGCGGTGAAATGCGTANAGATCTGGANGAATACCGATGGCGAANGCNGCCATCTGGGCCTAACACTGACGCTGANTGC GAAANCATGGGGGAGCAAACNNGATTANATACCCTGGTAGTCCATGCCGNAAACGATGTCTACTANNCGNTTGGGGCCTC TTGTANGCTTTTNNGGCGCNGCTTANCNNNNNNAATANNAACNNNCCCTNNNGGAGTANNGGTCGCNNNAANNNNNAACT NNCNNANTGAANTTGNNCGNGGGGNCCNCCNCAAANCNGGGTGGGGAGCANNNNGNNNNAATTTCNNATNGNNNNNCNNC CNNNNAAAANNNTTNNNNTGGGCNNNGTNNNNNNNNNNTANNAANNNTTTCNNNAAANNNNNATNNNGTTGGGNNNTCT Gram - cocci Y Microaerophile positive negative negative negative positive negative Y Unknown Had a gas bubble but zinc powder turned red which means no nitrate reduction Negative Positive, enzyme can break down peroxide Madeleine Hayman mhayman@gmail.com 2:00 PM B-13 Escherichia fergusonii 96.99 CATGCAGTCGTACNNNGTAGCNCNGTGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAAT TCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGACGCT CNNTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTG CCCTTGAGGCGTGGCTTCCGGGANCTAACGCGTTAAGTCGACCGCCTGGGGANTACGNCCGCAANGTTTAAAACTCAAAT GAATTGACGGGGGCCCCGCCNCAAGCGGTGGGAGCATGNGGNTTNATTTCGATGCAACGCGAAGAANNNTTACNTNGGTC TTGACNTCCACNGNAANNTTTCNNNNATGAAAAATGNTGCCNTT Gram - bacilli Y Falcultative anaerobe neagtive negative positive positive positive negative N N positive Negative positive for growth (gram neg) and positive for lactose fermenting positive Sam Boyd saboyd@scumb.edu 2:00 PM B-14 E. coli 95.86 GNNNACNNTGCAGTCGTANNNNNNNNNGCNNNNNNNNCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTG AGTAATGTCTGGGAAACTGCCCGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCA AAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAG GCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC AGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTA CTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCG TGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTA AGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGA ATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACG CTCANGTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT TGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGGAGTANGGCCGCAANGGTTAAAACTC AAATGAATTTGACGGGGGGGCCCGCACAAGCGGGTGGGAGCATGGNGGTTTAATTTCGNATGCAACGCGAAAGAANCNNT ACCCTGGNNNTTGACATCCCACGGGAAGTTTTTCAGANNATGANNNNGNGCCNTTCGGNAANCNGTGANACAGGGTGCNN GCATNNNTGNCNNTCAGCCTCCNGTGNTGNGAAANGNTGGGNNNNNAANNNNCGN Gram - Bacillus Y Facultative Anaerobe Negative Negative Positive Positive Positive Negative Y N No nitrate reduction Negative Gram -, lactose fermentor Positive Nikki Arevalo marevalo@csumb.edu 14:00 B-15 Acinetobacter calcoaceticus 85.53 GNTTANAGTTNATGNNGNAGACNTTAGAGCTGAATCTNATGGCCTATCCTGGTAACGAACTATGA ACAAGGTATCCTGACAACATTGTCGAGTCTAAATGTGAATGCTAATCCAGGTAACCTTATACGGGAGAAAGCAGGCGATC TTCTGACCTTGCGCTAATAGATGAACCTAATTCAGACTTCTACTTGGAGGGGTACAGGCCTACCTAAGCGACNATCTGTA TCAGTTCGGAGAAGATGATACTCCNCTCTGGGACTGTTACGCAGCCCTTACTCCTACGAGACGCGGCNGTGGGGAAGATT GGACAGAGGGCGCAAGCCTGATTCAGCAATGCCGCGTGTGTGAAGAAGGCCTAATGGCTGTATAACACTTTNANCGAGGA GGAGGCTNCTGCCTTTAATACCTTANNNATAGTGGACGTTACTCNCAGAATAANCACCGGTATAACTNTNNGCCAGCAGC CGCGGTAATACAGAGGGTGCNAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAATGTG ANATCCCCCNAGCTTAACTTGGGAATTGCATTCNATACTGNTTAGCTAGAGTGTGGGAGANGATGGTANAATTTCTNNGT GTAGTCGGTGANATGCGTAGAGATCTGNNAGGAATANNNATGGTNGNNNGCNGCCCNTCTNGNNCTAATNACTGNCNNCT GANGTGCGANNNCATGNNGNAGNNAAAC Gram - Bracillus Y Obligate Aerobe Positive: Tristian Lozano tlozano@csumb.edu 2:00 PM B-16 gram - Bacillus Y facultative anaerobe negative negative positive positive negative negative N negative gram -, lactose fermentor positive Zell Newton znewton-gardington@csumb.edu 10:00 AM F-1 TGCAGTCGTACAAGGTAACCGTANNNGCTGCTGATCATGTGCGNCGCTGCNATGANNGNGNG GNTGATTNTNTTNNTAGAGAGNATTGATGTCGAGTNNCCNAACANAATCNNCGCCCCTTGGTTTTGCTCGGANNNAANAA GANACACTACNNGTNGGGCGTGGCCAATCACGCCCNGCCTTTTCACCAATCCCAGCACGCCCCNTTCCGGATCGCCGCCA CCGCGATAGCCACTACTCGAGACCGGCAGCAAATAGAGGGGCCCNGGGCTCACCNNTCTTTCTTGCTACNCCCTTACGCA AAAACGCCTTGNNANACTTCAATGATACATCTGGAGCGGAGAANGAATG Gram - Bacillus Y facultative anaerobe Negative Negative positive Positive positive Negative N/A No capsule present Had no bubble but turned red after the drops were added.Able to convert nitrate to nitrile negative growth, gram -, lactose fermenter positive Leslie Pena lpena@csumb.edu 10:00 AM F-2 Gram + cocci Y Facultative Anaerobe negative negative negative negative positive yasmine cachos ycachos@csumb.edu 10:00 AM F-3 Escherichia E.coli 94.81 GCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACC TAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGANGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGNTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAAGCGGCCCCCTGGGACGAAGACTGACGCTCNNTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTTCCACGCCCGTAAACGATGTCGACTTGGGANNTGTGCCCNTGAAGCGTGGCTTNCNNGCTAACGCGTTTAAGTCGACCGCCTGGGGAGTANGNCNNNNNGNTAAAACTCAAATGAANTGANNGNGGCCCNNCNCAAGCCGGTGGNNNCATGNNGTTTAATTCNATNGCAACNCGAANAAANCNTTNNNCCTNGTCTTT Gram - Bacillus Y Facultative anerobe negative negative positive positive negative negative N reduction of nitrate to nitrite; reduction of nitrate negative gram -, lactose fermenter positive Mei Lin Bruder mbruder@csumb.edu 12:00 PM F-6 Acinetobacter calcoaceticus 97.64 TATGCACTTAACCAGGGGGACCGGGAGAGTAATGCTTATGAATCTGCCTATTANTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTANCGGGTCTGANAGGATGATCCGCCACACTGGGACTGATACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTATAGCACTTTAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCCTATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAANGCAGCCATCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTANCCGTTGGGGGCCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGG Gram - Cocci Y Obligate Aerobe Positive Negative positive negative negative negative N N Clear after zinc added negative positive positive Nicole Munoz nmunoz@csumb.edu 12:00 PM F-7 Caitlyn Swe cswe@csumb.edu 12:00:00 PM F-8 Gram - Cocci (change to bacillus) Y Facultative Anaerobe negative negative positive positive negative negative negative positive Fatima Leon-Garcia fleon-garcia@csumb.edu 12:00 PM F-9 N/A sample was not good, did not get any hits with the nucleotide sequence obtained. N/A N/A CATGNAGTCGTACAAGGTANCCNGTANNNNGTATANNAAAGAGAAGGGTAGAAATAAAAAAANTTT GGGATTNNTNATTANAAAANNNGGGATTTAANGNNACGGCNCCCNNAATNCCCNCCNNNNNNTNAGCGN Gram - cocci Y Facultative Anaerobe negative, green indicating no change in pH, Cannot use citrate as a source of energy negative, can't reduce sulfur Positive, Bacteria can split amino acid tryptophan to form the compound indole Positive, there was growth in and around where it was stabbed negative, no halo was formed meaning the unknown bacteria can use starch as a source of carbon and energy for growth. negative, no liquid was formed on the surface, bacteria cannot produce gelatinase. N/A N/A N/A N/A Positive, indicating bacteria can reduce nitrate to nitrite Negative, bacteria does not produce cytochrome C oxidases Positive, pink/red indicating that the bacteria is lactose fermenting N/A N/A positve, a lot of bubbles present, bacteria produces catalase enzyme Lilli Krier lkrier@csumb.edu 2:00 PM F-11 Escheria E.coli GCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTG AGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCA AAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCACCTAG GCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC AGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAANGNCTTCGGGTTGTAAAGTA CTTTCNGCGGGGANGAANNGAGTAAAGNTAATACCTTTNCTCATTGACGTTTACCCCGCAGAAGAANCACCGGGCTAACT CCGTGCCAGCAGCCGCGGTAATACNGAGGGTGCGAGCGTTAATCGGAATTTACTGGGCGTAAAGCGCACGCACGCGGNTT GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTANNGGGGGG GAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCCTGGNACGAAGA CTGANNCTCNNTGCGAAAGCGTGGGGGAGCAAACAGGATTAGATACCCTGGTAGTCNCACGCCCGTAAACGATGTCNACT TGGANTTGTGCCCTTGACGGCGTGNCTTCCCGGANCCTAACNCGTTAAGGTCGACCGCCTGGGGGANTNNGGGNCCGCNN GGTTAAAACTCAAAATGANATNNATNNGNNGGCCCCGNNNNNNNCCGNNGNAGCNTGNNGGNNNTTNNTTTNNAATGNNN ACGACGANNANNCATNAACCATGGNTNNTGNNNNTNCTNNCAGNANNNNNNANANNNTNNNNAAANGNNNNNCNTTCGGG NANNNNNGANANNNNGNCTNNCNNNNNNGTNCGNCCNNNCTCCNNGCT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive positive N/A(tube was lost) Durham tube= (+) for Nitrogen gas and (+) for Nitrate reducing to Nitrite Gram(-), lactase fermenting positive for catalase enzyme Erik Vaca evaca@csumb.edu 2:00 PM F-12 Bacillus wiedmannii 95.88% CTATACNTGCAGTCGAGCGATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTA ACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAATATTTTGAACTGCATG GTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTC ACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGA GGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGT AAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCT AACTACGTGCCAGCAGCCGCGGTAATACGTANGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGG TTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAGACTTGAGTGCAGAANAGG AAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAANGCGACTTTCTGGTCTGT AACTGACACTGAGGCGCGAAANCGTGGGGAGCAAACNNGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCT ANNTGTTANNNNNGTNTNCCGCCCCTTTTANTGCTGNAAGATTAATNGCATNNANGCACTCCTGCCNNGNNGGGAGTACN GGCCGCANNNCTGCANAACTNNNANNNATNGACGGGNGGGCNCCGCCACNNNNGNNGGNGCATGNNGGNNNTNAATTTNN NNAGCNNNNNCGANNNNNNTTNACTNANGNNNNTNNNCGATNCNTCTGCAAGAAACNCCNNNCNNANNATGANGNGNNNN NNNNNNNNNNNANNANNGAANNNNNNGNGCATN Gram + Cocci Y Facultative Anaerobe Negative negative negative negative negative postive Gas bubble present (+) ; Red color change (+) after addition of Reagents Positive Gram + , No lactose fermentation (-) positive for catalase enzyme Cassandra Majano cmajano@csumb.edu 2:00 PM F-13 Escheria E. fergusonii 95.76% ACATTGCAGTCGTANANNNNNNNNNNCNNNNNNNNCTTGCTTGCTTTGCTGACGAGTGGCGGACG GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAG ACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCAC CTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGG CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA AGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC TCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGG TAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACT GACGCTCNNTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGGA GGTTGTGCCCTTGNNNGTGGCTTCCCGGANCTAACGCGTTAAGTCGACCGCCTGGGGANTACGGCCGCNANGGTTAAAAC TCAAATGAATTGACGGGGGCCCCGCNNCAAGCNGNGGNANCATGNGNNTTAATTTCGATGCNACGCGNAAGAANNNTNAN CTGGGTCTTGACATCCACNGNAANNTTTTCANANATNGANNNNTGNTGCCCTTTCNGGNNNNNNNTNGANNAANNNNNGC NGGNCATNGGNNGTCGNNCANCNCNNNNT Gram - Bacillus Y facultative anaerobe Negative Negative Positive Positive Negative Negative Durham tube was postive for presence of Nitrogen gas and positive of reduction of NO2 Negative Gram (-) and is a Lactose Fermenter Positive for catalase enzyme Jill Castro jicastro@csumb.edu 2:00 PM F-14 Acinetobacter calcoaceticus 96.75% GCNNNCNTGNCNAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTANGAATCTGCCTATTANTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTANCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCANATAGTGGACGTTACTCNCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTANGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGANTTCCAGGTGTAGCGGTGAAATGCGTANAGATCTGGAGGAATACCGATGGCGAAAGCAGCCCTCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGGANNAAACAGGATTANATACCCTGGGTAGTCCNTGCCGTAAACGATGTCTACTANNNCGTTGGGGNCCTCTTNAGGCTTTTANTGGCGCNNCCTNNANNCGATATAATANAACNNNCCN Gram - Cocci Y Facultative Anaerobe Negative Ursula Lopez ulopez-blanca@csumb.edu 10:00 AM F-15 Escherichia E.coli 92.09% GCNANACATGCAGTCGTACAAGGTAGCCGTAAGNTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTNCNNGANGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCNTAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTANCTAGTAGGTGGGGTAACGGCTCACCAAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCATTGGNGAAGGGTGNAANNTGGGCGCAACCTGATGCCACCNTTCCGCGTGTATGAAAAANGCCTTCGGGTTGTATAGTACTTTTCAGCGGGGAGGAAGGGAGTACAATTAATA Gram - Bacillus Y Facultative Anaerobe: there was growth throughout the medium. This means that the bacteria can survive in high and low oxygen levels Negative: citrate agar plate did not have a color change (green). This means that the microbe was not able to use citrate for carbon and energy. The microbe did not accumulate alkaline/basic byproducts. Negative: no color change in the test tube. This means that the microbe was not able to reduce the sulfur in ferrous ammonium sulfate and sodium thiosulfate to produce hydrogen sulfide. Positive: color change at the top of the liquid in the test tube. This means that the microbe was able to produce indole from the amino acid tryptophan. Since indole was produced, the microbe reacted with a chemical reagent added after incubation to produce a color change. Positive: bacteria grew far away from the injection site and medium was hazy. This means that the bacteria was able to propel through the liquid by means of flagella. The microbe can swim by means of flagella. Negative: no halo in agar plate. This means that the microbe was not able to use starch as a source of carbon and energy for growth. Therefore, the microbe lacks the enzyme α-amylase Negative after 14 days: no liquification in the test tube. This means that the bacteria does not have the gelatinase enzyme. Since the bacteria does not have the gelatinase enzyme, it was not able to breakdown gelatin in order to utilize the amino acids. N No capsule present. There was a dark backrgound and purple stained bacterial cells were present. Positive: red after addition of A and B drops; no bubble present at top of Durham tube. Since the broth had a color change, it means that the unknown bacteria has denitifying capabilities and has a denitrifying enzyme. In the Durham tube there was no bubble which means that there was no reduction of N2. Negative: no color change on Sigma Aldrich strip. This means that the unknown bacteria does not contain the cytochrome c oxidase enzyme. Therefore, the unknown bacteria is not capable of aerobic respiration by having oxygen be the final electron acceptor in the electron transport chain. Gram - bacteria: pink growth on agar plate. This means that the bacteria was able to ferment lactose Negative: no bubbles on microscope slide. This means that the microbe does not have the catalase enzyme which is capable of catabolizing hydrogen peroxide into H2O and O2. Allexxis Hand ahand@csumb.edu 2:00 PM F-16 Escherichia fergusonii 95.98% CATGCAGTCGTANANNGNNNNGCGCNNNNNNNNGCTTGCTTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCNCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTC ACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGT AAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGT TTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTATAGATCTGGAGGAATACCGGTGGCGAAAGCGGCCCCCCTGGACGAAN ACTGACGCTCANGTGCGAAAGCGTGGGGNGCAAACACGNATTANATACCCCTGGNNAGTCCACGCCGTAAACGATGTCNACTTTGGNAGGTTGTGCCCCTTGNNNGTGGCTTCCGGANCTAACNCGTTNNNNCNNCCGCCTGGGGANTANNNCGCNNNNN NNNACTCTNATGNATTGACNGNGGGNCCCNCACAAGCNGNNGGGANNATGTGGNTTTNATTCGAATNGCNACNCGAANNNNNTTACCTNNNNTTTGNACATCNCACNNAANNTTT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive negative Negative after 14 days Possible endospores or it was just really burnt No Positive. After 8 drops of Reagant A and B it turned pink so bacteria can reduce nitrate into nitrite. Negative. Gram negative and lactose fermenting Positive Joseph Hanson jhanson@csumb.edu 2:00 PM M-1 NNNNNNNNNNNNNNNNNNNNNNNNNNANNGGGGTANNGNAAATAGTTTATTTTTTGAGNGGGNGGGGAGGTTCTTCTTTA TTGCCACTCCTNNNNAGACGCNNGACNGNNNNGCTACCCATNCCNTNTCCGCANAGACCNNNANGACTTCCTACGGNTCC TGACTCATGAGGCTNCCTGGGAGATTGTNGGNNTAGGCTANCNGNCCAAGANAATTGCNCCGNGGNATATGANAAGNACA CCNTCANGGTGAANTGACCCCCGGCCCACTCCCACGNCGNNAGCCGNGCGNGAAGTTTGGGACGGANGTTNNNNNNNTAC GANNNNCGATGNANNNNGAAAGGTTAGTGTNCATCTNCTGGGCCGAGGGACCGGGANGANTTTTCATTNNNTAANGGACG CCCGCANAAAAAGCCCTNCTTTTCTGCCCCCACNACANNTAGGANGCGTAATCTNTTTGTATTCTGAACTAAGCCNCCGG GAAN gram - cocci N facultative anaerobe negative, microbe cannot use citrate for carbon and energy negative, bacteria cannot reduce sulfur in ferrous ammonium sulfate / soidum thiosulfate to hydrogen sulfide positive, bacteria produces indole from tryptophan positive, bacteria can swim by flagella negative, bacteria cannot hydrolize starch negative, bacteria does not have gelatinase enzyme thus cannot break down gelatin or uitilize its amino acids Miranda Iturriaga miturriaga@csumb.edu 10:00 AM M-2 Acinetobacter Calcoaceticus 94.04% GGGGTCGATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTAYGAATCTGCCYMTTAGTGGGGGACAACATTTCGAARGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAKACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACGTTTACTCGCARAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAAWTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTATARATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAACAACTGACGCTGAAGGTGGCGGAA Gram - Cocci N Obligated Aerobic Positive Negative Negative Negative Negative Negative Negative Negative Gram -/lactose fermentor Positive Michael Everton meverton@csumb.edu 10:00 AM M-3 Acinetobacter Lactucae GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram + Bacillus N Faculatative Aerobic positive negative negative negative N Andrea Rios andrios@csumb.edu 10:00 AM M-4 Acinetobacter Acinetobacter calcoaceticus GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram - cocci Melissa Meraz memeraz@csumb.edu 12:00 PM M-7 Acinetobacter GGGGTCTATTCGTAACCGGGGAGCCGTGAGAGTAATGCTTANGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCANACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAAGCGAGGAGGAGGCTACTGAAGTTAATACCTTTCAGATAGTGGACCTTTACTCGCANAATTAAGCACCGGCTTTACTCTGGTGCCAGCAGCCGCGGTAATACAGAAGGTGCCAGCGTTTAATCGGATTTACTGGGCGTAAAACGCGCGTAGGCGGCTTATTAAANTCAAATGTGAAATCCCCCCAGCTTAACTTGGGGAATTGCATTCTATACTGGTTAGCTAGAGTGTGGGGAGAGGATGGTANAANTTCCACGGTGGTAGCGGTGAAATGCGTANANATCTGGGAGGGAATACCGATGGGGGAAGGCAGCCCTCTGGGCGCTAAC Gram - Cocci Y Facultative anaerobe Positive Negative Negative Positive Negative Negative N N Negative Negative Gram - and lactose fermentor Positve Guadalupe Salamanca gsalamanca@csumb.edu 12:00 PM M-8 NNNNNNNGNNACNNTGCAGTCGTACAGGTANCCGTANNTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCT Gram - Bacillus Y Facultative Anaerobic Negative Negative Positve Positive Negative Negative Negative Positve Positive Marin Medina marimedina@csumb.edu 12:00 PM M-9 Escherichia E. Coli 97.32% GGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA (ACCNTGCAGTCGTACAGGTAGCCGTAACTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCT GGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA CCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCC CTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGG GGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGC CGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGT GAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG TAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCG AAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGA GGCGTGGCNTTCCGGANCTAACGCGTTAAGTCGACCGCCCTGGGGGAGTACNNCCGCAANGTTTAAAACTCNNATGAATT TGACGGGGGGCCCCGCCNCNAAGCCGGNNGGANCATGTGGNTTAATTTCNATGC) Gram - Bacillus Y Facultative anaerobe negative negative negative positive positive Negative N No Capsule present Negative Natalie Ward nward@csumb.edu 2:00 PM M-11 Acinetobacter sp. calcoaceticus 90.79% CCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCC gram - cocci Y facultative anaerobe positive (blue) negative, no H2S produced negative negative negative Negative; cannot digest gelatin for carbon and energy source N No Capsule present Positive; Bubble in durham tube(possible nitrate), no color change after reagents, red after addition of zinc (no nitrate reduction) Negative Positive Gram - growth and Lactose fermentation Positive for catalase enzyme Antonio Hernandez ahernandez-sanchez@csumb.edu 2:00 PM M-12 Acinetobacter calcoaceticus 97.29% GCAAGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTA Y Facultative anaerobe Positive(blue color) negative negative negative negative Negative Positive Jakobia Brown Jakbrown@csumb.edu 10:00 AM M-13 Escherichia E.coli 86.82% GCNNNNCATGCAAGTCGTACNNNNNNNNNCNNNNNNNNNCTTGCTGCTTTGCTGACGAGTGGCGGACGGGT GAGTAATGTCTGGGAAACTGCCCGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACC AAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTA GGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAG CAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT ACTTTCAGCGGGGGAGGGAAGGGGANNTAAAGTTAATANCCTTTTGCCTCATTGGNACGTTNCCCCGNNAGAAANAAGGC ACACCGGCTNNACTCTCCNNTCCNNNCAAGCCCGNCGGTAAATANCNGGAGAGGTGTGCNAGCCGTTAATCNGGAAANTN NNNGGGGCNNNAAAANCNNCNCNCNNGGCGGGTTTGTTAAGTCAGAATGTGAAATCCCCCCGGGCTTCANCCTGGGGAAC TGCCTCNTGATACTGGGCNAGCTTGAGTCTCCGTAGANGGGGGGNAGAATTCCCNGGTGTTAGCGGTGNNAATGCGTANA NATCTGGGAGGAATACCGGNGGNGAANGCGGCCCCCTGGNCGAAANANTGACGCTCNNGTGCGNAAANNGTGNNGGAGGC AAACANGGATTANATNACCCCTNNGNNAGTCCACGCCCGCNNNANNANGNTCNNACNTAGTGANGGNTGTTGTCCCCNNN NNNNGCGGNNNNTGTCCNNGNNNNNNNAACNNNNNTNNNNNNTNCNNCCNNNCCCTGNNNNGNANNNNNNNNNCCCNNCC NNNNNNTNNNNNANCNTNCNCNANNNNANNNNNNNNNGGNGGNNNNNNNNNNNNGNNNGGNNNNANNNNGNNNNNNTNNN NTGNNANNNNCNNANNNNNNTNCNTNGNNNNNNNNNNNNNCCNNCNNNCANNNNTTC Gram - Bacillus Y Facultative Anaerobe Negative (Green) Negative Positve positive Positive Negative N/A N/a Positive: The tube had a bubble so it is a denitrifying bacteria Positive: uses oxygen as the final electron acceptor Gram negatvie and was pink meaning that it has lactose fermenting colonies Positive- Bacteria bubbled Kendall DeRosa kderosa@csumb.edu 2:00 PM M-14 AGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGA Carlos Rosas crosas@csumb.edu 14:00 M-15 CGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTG Gram - Bacillus Y Facultative anaerobe negative negative negative negative negative positive Brianna Cabrera bcabrera@csumb.edu 2:00 PM T-1 Shigella S. dysenteriae 92.01% GGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTT Gram - Bacillus Y Facultative Anaerobe negative negative positive positive negative negative N Able to reduce Nitrate to Nitrite Negative Gram (-), ability to ferment lactose Negative Evan DesJardins edesjardins@csumb.edu 10:00 AM T-2 AAGCGAGGAGGAGGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGC Gram + Bacillus Y facultatice anaerobe negative negative positive positive negative negative Positive Gram-,lactase Peter Nelson pnelson@csumb.edu 10:00 AM T-3 CAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGT Gram - cocci Y Facultative anaerobe negative Rachel Durand rdurand@csumb.edu 2:00 PM T-4 Acinetobacter calcoaceticus 96.58% CAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATT Gram - Bacillus Obligate Aerobe negative negative positive positive negative negative Negative Positive Alyssa Ruiz alyruiz@csumb.edu 10:00 AM T-5 CCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGATGGCGAANNCAGCCATCTGGCCTAACACTGACGCTG Gram - Cocci Y Facultative Anaerobe negative negative positive positive negative- unable to digest starch for carbon and energy source negative- no gelatinase enzyme, unable to use gelatin as carbon and energy source NA- was unable to complete test NA- unreadable under the microscope positive- bacteria has denitrifying capabilities, reduction to N2 Negative positive, gram - and can ferment lactose negative- not capable of catabolizing hydrogen peroxide Grace Fairbourn gfairbourn@csumb.edu 12:00 PM T-6 Acinetobacter calcoaceticus ANTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCCGTTGGGG gram - cocci Y - can survive without oxygen Facultative Anaerobe - can survive in high and low oxygen positive - can utilize citrate as carbon and energy negative - cannot reduce sulfur negative - does not produce indole from trypotophan negative - cannot freely move around negative - cannot digest starch for source of carbon and energy negative - cannot digest gelatin for carbon and energy source negative - cannot reduce nitrate at all Negative - does not have cytocrome C oxidase enzyme lactose fermenting gram - positive - can safely breakdown H2O2 Raiya Kimble Rkimble@csumb.edu 12:00 PM T-7 NCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGGAGTACNGTCGCANACTAAAACTCAAATGA gram - Bacillus Facultative anaerobic- no growth at the top of broth negative negative Positive Positive positive Negative Negative Positive, gram - Stephen Nelson Stnelson@csumb.edu 12:00 PM T-8 Acinetobacter lactucae 93.59% ANTTGACGGGGGNCCNCCNCNAGCGGNGGNNCATGNNGNTTAATTCGATGCNNCGCGAANAANNTTANCNNGG Gram - Cocci 0.5 micrometer diameter Y Facultative Anaerobe Positive Negative Positive Positive Negative Negative after 14 days No endospores evident in staining - Also, species is cocci which are not known to produce endospores No capsule present Negative Lactose-fermenting, Gram - Positive for catalase production Nathan Gyan ngyan@csumb.edu 12:00 PM T-9 Escherichia Escherichia coli 92.35% TGMAKTCGDAVRRGGTAGSCGTAACTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACCTAGGCGACGAYCCCTASCTGGTCTGAGAGGWKGACCAGCCACACTGGAACTGARACACGGTCCARACTCCTACGGGAGGCAGCAKTGGGGAATWTTGCMCAATGGGCGCAAGCCTGWDGCASCCWTGCCGCGTGTATGAARAAGGSCTTCGGGTTGDAAAGTACTTTCVGCGGGGARGAAGGGARDAAAGKTAATACCTTTGCTCHTTGACGTTHCCCGCARAARAAGCACCGGCTAACTCCGKGCCAGCAGCCGCGGTAATACGGAGGGKGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCMCGCAGGCGGTTTGTTAADTCAGAWGTGAAATCCCCGGGCTCAACCTGGGAACTGCHTCTGATACTGGCAAGCTTGAGTCTCGTARARGGGGGKAGAATTCCVGGKGTAGCGGKGAAATGCGTARAGATCTGGAGGAATACCGGBGGCGAAGGCGGCCCCCTGSACGAANACTGACGCTCANGTGCGAAAGCGTGGGGGAGCAAACAGGATTANATACCCTNGNTAGTCCMCGCCGTAAACVATGTCGACTTGGAGGTTGTGCCCTTGANGCGTGGCTTCCGGNAGCTAACGCNTTTAAGTCGACCGCCTGGGGGAGGTANGNCCGCAAGGTTAAAACTCAAATGAATTTGACTGGGGGGCCCNGCCNCAAGCCGGTGGANCNTGTNNTTTAATTTCGATGGCAA Gram - Cocci Y Facultative Anaerobe Negative Negative Positive Positive Negative Negative Negative Roman Maaske rmaaske@csumb.edu 2:00 PM T-11 Gram- Bacillus Y facultative anaerobe Mylisa King myking@csumb.edu 2:00 PM T-12 CNNTGCAGTCGTANANNNNNNNNGCNNGTNNAGCTTGCTGCTTTGCTGACGAGTGGCGGACGGG TGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGAC CAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAAGGCTCACCT AGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA GCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG TACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTC CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGT TAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTA GAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAANGCGGCCCCCTGGACGAAGACTGA CGCTCNGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGGAGG TTGTGCCCTTGANNCGTGGGCTTCCCGGANCTAACGCGTTAAGTCGACCCGCCCTGGGGAGTANGNCCGCAAGGTTAAAA CTCAAATGAATTTGACGGGGGNCCCGCCNCAAGNCGGTGGANCATGGNGGNNNATTTCGATGCNNNCNCGAANAANNNTT ACCTNGTCNTGNCNTCCANNNNNGTTTTCNNANATGNNAATGTGNCNTTNNNNNNNCNNNGANNANNNNNNNNNGCATNN NTNNTCGTCAGCCTCC Gram - bacillus Y Facultative anaerobe Negative Negative Positive Positive Negative Negative @ day 7 Y Not present 3 bubbles, Red after Reagent A & B Negative Lacatose fermentor (pink in color), Gram- negative (growth present) Positive Alexa Blunt alblunt@csumb.edu 14:00 T-14 Acinetobacter Acinetobacter lactucae 97.81% ACATGCAGTCGAGCGGAGTGANGGTGCTTGCACTATCACTTAGCGGCGGACGGGAGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTATGGTTGTAAAGCACTTTAAGCGAGGAGGAGGCTACTTTAGATAATACCTAGAGATAGTGGACGTTACTCGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGCTAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGNNTGCGTAGAGATCTGGAGGAATACCGATGGCGAAGGCAGCCATCTGGCCTAACACTGACGCTGANGTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCGTTGGGGCCTTTGAGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGANCATGNNGGTTTAATTTCGATGCANCGCGAAGAANCCTTTANCCTGGNCNTGACNTANTAAGAACTTTTCCCANNANNATGGGAATTGGNGNNTTNNNNNNTTACATACAGNGCTGCATGNTGTCGTCNNCTCNNGNCGNGANAT Gram - cocci Y Facultative Anaerobe Positive Negative Negative Negative Negative Negative after 14 days Possible endospore, green stained Negative Color change after gas bubble present and addition of Reagents A and B Negative Negative Lactose fermentation, pink with no pink cloud, positive Kendall DeRosa kderosa@csumb.edu 2:00 PM m-16 Acinetobacter Acinetobacter calcoaceticus 93.15 TNACCNTGCNGTCGAGCGGAGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGGTGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACATTTCGAAAGGAATGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTAATAGATGAGCCTAAGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGGATCCAGCCATGCCGCGTGTGTGAAGAAANNCTTATGGNTGTAAAGCACTTTAAGCGAGGANGANGCTACTGAAGTTAATACCTTCAGATAGTGGACGTTACTCGCANAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGANGGTGCAAGCGTTAATCNGATTTACTGGGCGTAAAGCGCGCGTNNCGGCTAATTAAGTCAAANGTGAAATCCCCNAGCTTAACTTGGGGAATTGCATTCGATACTGGTTAGCTAGANTGTGGGGAGANGATGGTAGAATTCCANGTGTAGCGGTGAAATGCGTAGAGATCTGGANGAATACCGATGGCGAAAGCAGCCATCTGGGCCTAACACTGACNCTGAGTGCGAAAGCATGGGGAGCAAACANGGATTAGATACCCTGGGTAGTCCATGCCCGTAAACGATGTCTACTANCCNTTTGGGGCCTTTGNNNCTTTANTGGCGCAGCCNAACNCCNATNAAGTAANAACCGCCNNGGGGGCANGTACGGGTTCGCNNNACTAAAACTCNAATNNANTTGANNGGGGGNCCCGCCNNCNNNCCNNTGGNANCATGNTGNNTTTAATTTCNATNNNNNNCCNCNNNNAACCNNNANCCTNGNNNNTGNCNTNNGTAANNANNTNNNNNANNNNNNNNNNNNNNCGGAACTTNNANTNCNGNNCCTGCAT Gram -

Related Questions

Find Related Questions Here